KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/27341
Title: | Production of sheep embryos in vitro |
Other Titles: | Not Available |
Authors: | Mor A, Mondal S and Reddy IJ |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::National Institute of Animal Nutrition and Physiology |
Published/ Complete Date: | 2017-07-24 |
Project Code: | Not Available |
Keywords: | Oocytes; Sheep; In vitro maturation; In vitro fertilization; Embryo |
Publisher: | Austin publishing group |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | In-Vitro Maturation (IVM) and In-Vitro Fertilization (IVF) are the potential methods for producing the early embryos in large numbers. Recently many producers have introduced IVF into their reproductive programs. Successful in vitro production of embryo depends upon the good oocyte maturation and correct sperm capacitation. Ovaries were collected from non-pregnant slaughtered sheep from a local slaughterhouse in normal saline. Ovaries having visible follicle and granular homogenous ooplasm were selected and cultured in maturation medium at 38.5º C, 5% CO2 and 95% humidity for 22-24 hr. The degree of cumulus cell expansion was determined after 22-24 hr of IVM and oocyte with expanded cumulus cell mass to at least 2 diameters away from the zona pellucida were considered as cumulus expanded. The matured oocytes were inseminated with 1 to 2 million spermatozoa/mL in Brackett and Oliphant medium and the embryos were then cultured in CO2 incubator at 38.5° C, 5% CO2 and 95% humidity and the cleavage was checked after 42-48 hr post insemination. The embryos were then further cultured for 6-7 days in order to produce a complete developmental series from the 2 cell to blastula stage of embryonic development. Our results showed that the percentage of maturation was 81.81±1.10, cleavage rate was 66.03±1.33, 8-16 cells was 43.44±0.62, Morula was 20.01±0.29, and Blastula was 8.59±0.56. The maturation rate was significantly (P<0.05) higher in May as compared to January, February, March and April. The percentage of 8-16 cell stage was also significantly higher in May as compared to those during February. It is concluded that the cleavage rate was good with Brackett and Oliphant fertilization media and TCM 199 as culture media in sheep with high blastula percentage during January. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Article |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Austin Endocrinology and Diabetes Case Reports |
Volume No.: | 2(1) |
Page Number: | 1011 |
Name of the Division/Regional Station: | Animal Physiology Division |
Source, DOI or any other URL: | http://austinpublishinggroup.com/diabetes-case-reports/download.php?file=fulltext/diabetescr-v2-id1011.pdf |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/27341 |
Appears in Collections: | AS-NIANP-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.