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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/27970
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Dey, P., Biswas, C. and Karmakar, P.G. | en_US |
dc.date.accessioned | 2019-12-05T08:28:59Z | - |
dc.date.available | 2019-12-05T08:28:59Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | Not Available | en_US |
dc.identifier.issn | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/27970 | - |
dc.description | Not Available | en_US |
dc.description.abstract | A RIL population of jute developed by crossing one resistant accession CIM 036 and a susceptible variety JRC 412 was used to identify novel defence related miRNAs activated upon challenged inoculation with stem rot pathogen Macrophomina phaseolina. About a total of 15.7 million reads were generated from the resistant line with the read length of 1 × 50 bp. Here, we identified nine mature novel microRNAs which passed Minimum Free Energy (MFE Kcal/mol) criteria. Target site and secondary structure were predicted and most of them showed ubiquitination and selective autophagy activity with high expression value. Five novel miRNAs viz. Candidate_41, Candidate_9, Candidate_66, Candidate_65 and Candidate_8 had free energy less than -25 kcal/mol. Known microRNAs viz. miR-845b and miR-166 superfamily are abundantly expressed with high expression value. The sequence of jute miR-845b superfamily is identical to that of Arabidopsis thaliana except at 18th position, but unlike in A. thaliana it targets the coding sequence for the P-loop motif in the mRNA sequences for disease resistance proteins with nucleotide binding site (NBS) and leucine-rich repeat (LRR) motifs. In-silico analysis suggested that miR-845b and miR-166 superfamily provided NBS-LRR and ROS mediated defence and subsequently expression of novel microRNAs with selective autophagy activity enabled multi-layered defence cascade against M. phaseolina in jute. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Not Available | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | miRNAs, resistant RIL line | en_US |
dc.subject | jute | en_US |
dc.title | Identification and characterization of differentially expressed novel miRNAs (21–24 nt) in a Macrophomina phaseolina resistant RIL line of jute (Corchorus capsularis L.) | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Physiological and Molecular Plant Pathology | en_US |
dc.publication.volumeno | 94 | en_US |
dc.publication.pagenumber | 62–66 | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | Not Available | en_US |
dc.publication.authorAffiliation | ICAR::Central Research Institute for Jute and Allied Fibres | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.naasrating | 7.65 | en_US |
Appears in Collections: | CS-CRIJAF-Publication |
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