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http://krishi.icar.gov.in/jspui/handle/123456789/27996
Title: | A highdensity intraspecific SNP linkage map of pigeonpea (Cajanas cajan L. Millsp.) |
Other Titles: | Not Available |
Authors: | Arora, S., Mahato, A.K., Singh, S., Mandal, P., Bhutani, S., Dutta, S., Kumawat, G., Singh, B.P., Chaudhary, A.K., Yadav, R., Gaikwad, K., Sevanthi, A.M., Datta, S., Raje, R.S., Sharma, T.R., Singh, N.K. |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR-CRIJAF |
Published/ Complete Date: | 2017 |
Project Code: | Not Available |
Keywords: | highdensity intraspecific,SNP linkage map,pigeonpea,Cajanas cajan L. Millsp. |
Publisher: | Not Available |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | Pigeonpea (Cajanus cajan (L.) Millsp.) is a major food legume cultivated in semi-arid tropical regions including the Indian subcontinent, Africa, and Southeast Asia. It is an important source of protein, minerals, and vitamins for nearly 20% of the world population. Due to high carbon sequestration and drought tolerance, pigeonpea is an important crop for the development of climate resilient agriculture and nutritional security. However, pigeonpea productivity has remained low for decades because of limited genetic and genomic resources, and sparse utilization of landraces and wild pigeonpea germplasm. Here, we present a dense intraspecific linkage map of pigeonpea comprising 932 markers that span a total adjusted map length of 1,411.83 cM. The consensus map is based on three different linkage maps that incorporate a large number of single nucleotide polymorphism (SNP) markers derived from next generation sequencing data, using Illumina GoldenGate bead arrays, and genotyping with restriction site associated DNA (RAD) sequencing. The genotyping-by-sequencing enhanced the marker density but was met with limited success due to lack of common markers across the genotypes of mapping population. The integrated map has 547 bead-array SNP, 319 RAD-SNP, and 65 simple sequence repeat (SSR) marker loci. We also show here correspondence between our linkage map and published genome pseudomolecules of pigeonpea. The availability of a high-density linkage map will help improve the anchoring of the pigeonpea genome to its chromosomes and the mapping of genes and quantitative trait loci associated with useful agronomic traits. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | PLOS One |
NAAS Rating: | 8.74 |
Volume No.: | 12(6) |
Page Number: | e0179747 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/27996 |
Appears in Collections: | CS-CRIJAF-Publication |
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