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Title: | Effect of in ovo inoculation of vitamin E on expression of Hsp-70 mRNA and juvenile growth in coloured broiler chicken |
Other Titles: | Not Available |
Authors: | Rajkumar U., Vinoth A., Rajaravindra K.S., Shanmugham M., Rao S.V. Rama |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR-Directorate of Poultry Research |
Published/ Complete Date: | 2015-01-01 |
Project Code: | Not Available |
Keywords: | Hsp-70, vitamin E |
Publisher: | IPSA |
Citation: | Rajkumar U.*, Vinoth A., Rajaravindra K.S., Shanmugham M., Rao S.V. Rama. 2015. Effect of in ovo inoculation of vitamin E on expression of Hsp-70 mRNA and juvenile growth in coloured broiler chicken |
Series/Report no.: | Not Available; |
Abstract/Description: | The effect of in ovo inoculation of vitamin E (Vit E) at 18th day of incubation on expression of Hsp-70 mRNA and body weight during post natal life at 6 weeks of age was studied in PB-2, a coloured broiler parent line. A total of 108 fertile eggs, 18 for each treatment were inoculated with varying concentrations of Vitamin E (a-tocopherol) with sham and un-injected control. The body weights at different Vit E concentrations were not significant at day old, 2nd, 4th and 6th weeks of age. The hatchability did not vary significantly among the sham control, normal control and treatment groups. The expression of Hsp-70 mRNA varied significantly (P≤0.05) between the tissues. However, varying concentrations of in ovo inoculation of Vit E had no significant (P=0.968) effect on m RNA expression. The interaction between treatment and tissue was not significant (P=0.257). The mRNA expression ranged from 40.62 in control to 40.0 in 25 μg treatment. The Hsp-70 mRNA expression in heart and liver was 42.93±2.38 and 41.76±1.87 in relation to the internal control GAPDH expressed in terms of 40-”Ct. The Hsp-70 mRNA expression was significantly (P>0.05) higher in heart and liver compared to the other tissues. Muscle expressed significantly (P>0.05) low levels of Hsp-70 mRNA. The study concludes that, in ovo inoculation of VitE has revealed positive trends though not significant in terms hatchability, growth and Hsp-70 gene expression. The Hsp-70 mRNA expression was significantly higher in heart and liver, the important organs involved in stress management in birds. The dose of Vit. E was not sufficient to elucidate any significant effect on Hsp-70 m RNA expression, which needs further studies with higher dosage to determine the optimum level of Vit E. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Article |
Sponsors: | iCAR-DPR |
Language: | English |
Name of Journal: | Indian Journal of Poultry Science |
NAAS Rating: | 5.85 |
Volume No.: | 50 (1) |
Page Number: | 104-108 |
Name of the Division/Regional Station: | Poultry Genetics and Breeding |
Source, DOI or any other URL: | https://www.indianjournals.com/ijor.aspx?target=ijor:ijps&volume=50&issue=1&article=020 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/28077 |
Appears in Collections: | AS-DPR-Publication |
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