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http://krishi.icar.gov.in/jspui/handle/123456789/28172Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Sanjay Kumar, Rajender Kumar, Ashok K. Gupta, Suresh C. Yadav,Sachin K. Goyal, Sandip K. Khurana, Raj K. Singh | en_US |
| dc.date.accessioned | 2019-12-05T11:03:42Z | - |
| dc.date.available | 2019-12-05T11:03:42Z | - |
| dc.date.issued | 2013-08-30 | - |
| dc.identifier.citation | Not Available | en_US |
| dc.identifier.issn | Not Available | - |
| dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/28172 | - |
| dc.description | Not Available | en_US |
| dc.description.abstract | tEquine piroplasmosis is a tick-transmitted protozoan disease caused by Theileria equi and/orBabesia caballi. In the present study, we expressed a 53 kDa protein from the truncated EMA-2 gene of T. equi (Indian strain) and developedEMA-2ELISA using this expressed protein. ThisELISA is able to detect T. equi-specific antibodies in experimentally infected animals as earlyas 9 days post-infection. The assay developed was validated with the OIE recommendedcompetitive ELISA (cELISA) on 120 serum samples and significant agreement (kappa = 0.93)was observed between results of both the ELISAs which indicates suitability ofEMA-2ELISAfor use in sero-diagnosis. Diagnostic sensitivity and specificity ofEMA-2ELISA – as comparedwith cELISA – were 0.97 and 0.96, respectively. Analysis of 5651 equine serum samples– collected during 2007–2012 from 12 states of India representing eight agro-climaticzones – byEMA-2ELISA revealed 32.65% seroprevalence of T. equi in India. In conclusion, theEMA-2ELISA developed using the T. equi EMA-2 recombinant protein as antigen for detectingT. equi-specific antibodies has good diagnostic potential for sero-epidemiological surveys. | en_US |
| dc.description.sponsorship | Not Available | en_US |
| dc.language.iso | English | en_US |
| dc.publisher | Elsevier | en_US |
| dc.relation.ispartofseries | Not Available; | - |
| dc.subject | Babesia equi, Equine piroplasmosis, ELISA EMA-2, Seroprevalence, Theileria equi | en_US |
| dc.title | Development of EMA-2 recombinant antigen basedenzyme-linked immunosorbent assay for seroprevalencestudies of Theileria equi infection in Indian equine population | en_US |
| dc.title.alternative | Not Available | en_US |
| dc.type | Book | en_US |
| dc.publication.projectcode | Not Available | en_US |
| dc.publication.journalname | Veterinary Parasitology | en_US |
| dc.publication.volumeno | 198 | en_US |
| dc.publication.pagenumber | 10-17 | en_US |
| dc.publication.divisionUnit | Not Available | en_US |
| dc.publication.sourceUrl | oi.org/10.1016/j.vetpar.2013.08.030 | en_US |
| dc.publication.authorAffiliation | ICAR::National Research Centre on Equines | en_US |
| dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
| dc.publication.naasrating | 8.16 | en_US |
| Appears in Collections: | AS-NRCE-Publication | |
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