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http://krishi.icar.gov.in/jspui/handle/123456789/28689| Title: | Genetic Analysis of the Neuraminidase (NA) Gene of Equine Influenza Virus (H3N8) from Epizootic of 2008–2009 in India |
| Other Titles: | Not Available |
| Authors: | B.C.Bera Nitin Virmani |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::National Research Centre on Equines |
| Published/ Complete Date: | 2013-05-24 |
| Project Code: | Not Available |
| Keywords: | equine influenza |
| Publisher: | Not Available |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | The neuraminidase (NA) gene sequences of four Indian equine influenza viruses (EIVs) isolated from epizootic in 2008 and 2009were analyzed. The phylogenetic relationship and selection pressure of NA genes were established in comparison to other EIVs circulating worldwide along with the domains and motifs of the encoded protein to find out the significance of mutational changes. Among Indian isolates, two amino acid (aa) changes each in Mysore/12/08 (Asn67Tyr & Asp396Gly), Gopeshwar/1/09 (Ile49Val & Asp396Gly), and Uttarkashi/1/09 (Ile49Val & Asp396Gly) isolates were observed in respect to Jammu-Katra/06/08 isolate. Amino acid (aa) sequence analysis also revealed five consistent aa residue changes viz, Gly/Arg40Glu, Tyr66His, Val191Ile, Val209Ile and Asp235Asn in Asian including Indian isolates, Spain/07 and Spain/09 isolates in comparison to other EIVs circulating worldwide. The topology of the phylogenetic tree revealed that the Indian, Chinese, Mongolian and Kazakhstan isolates together formed a subgroupwith Yokohama/10 isolate. Spain/07& Spain/09 isolates showed closest clusteringwithAsian isolates. This indicates that non-synonymousmutations in Asian isolates with temporal pattern originating from Spain/07, led to the subgroup of the Asian isolates within Florida clade 2 sublineage. The analysis of the predicted secondary structure has not shown any significant difference in the NA proteins of all Indian isolates. Fixed-effects likelihood (FEL) analysis of the selection pressure revealed three codons (43, 355 & 434) under positive selection pressure. The overall evolutionary changes (x value) of 3.4 indicatesNA gene to be under strong selection pressure. Further, seven putative N-glycosylation siteswere observed in theNAprotein.Themapping of specific aa changes, theirmutational and functional analysis need to be carried out to ascertain their role in pathogenecity of the virus. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Article |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Virus Disease (Indian Journal of Virology) |
| NAAS Rating: | 5.95 |
| Volume No.: | Not Available |
| Page Number: | Not Available |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | DOI 10.1007/s13337-013-0137-0 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/28689 |
| Appears in Collections: | AS-NRCE-Publication |
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