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http://krishi.icar.gov.in/jspui/handle/123456789/29384
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kumar N | en_US |
dc.contributor.author | Bhatia S | en_US |
dc.contributor.author | Pateriya AK | en_US |
dc.contributor.author | Sood R | en_US |
dc.contributor.author | S Nagarajan | en_US |
dc.contributor.author | Murugkar HV | en_US |
dc.contributor.author | Kumar S | en_US |
dc.contributor.author | Singh P | en_US |
dc.contributor.author | Singh VP | en_US |
dc.date.accessioned | 2020-01-08T06:12:07Z | - |
dc.date.available | 2020-01-08T06:12:07Z | - |
dc.date.issued | 2019-09-25 | - |
dc.identifier.citation | Kumar N, Bhatia S, Pateriya AK, Sood R, S Nagarajan, Murugkar HV, Kumar S, Singh P, and Singh VP. (2019). Label-free peptide nucleic acid biosensor for visual detection of multiple strains of influenza A virus suitable for field applications. Analytica Chimica Acta, 1093: 123-130. | en_US |
dc.identifier.issn | 2590-1346 | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/29384 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Accurate and rapid diagnosis of Influenza A viruses (IAVs) is challenging because of multiple strains circulating in humans and animal populations, and the emergence of new strains. In this study, we demonstrate a simple and rapid strategy for visual detection of multiple strains of IAVs (H1 to H16 subtypes) using peptide nucleic acid (PNA) as a biosensor and unmodified gold nanoparticles (AuNPs) as a reporter. The design principle of the assay is based on the color change on account of free PNA-induced aggregation of AuNPs in the presence of non-complementary viral RNA sequence and vice-versa. The assay could detect IAV RNA with a visual limit of detection of 2.3 ng. The quantification of RNA with a considerable accuracy on a simple spectrophotometer was achieved on plotting the PNA-induced colorimetric changes (absorption ratio of A640/A520) in the presence of a varying concentration of complementary RNA. As a proof-of-concept, the visual assay was validated on 419 avian clinical samples and receiver operating characteristic (ROC) curve analysis showed a high diagnostic specificity (96.46%, 95% CI = 93.8 to 98.2) and sensitivity (82.41%, 95% CI = 73.9 to 89.1) when RT-qPCR was used as reference test. Hence, the simplicity, rapidity, and universality of this strategy make it a potential candidate visual assay for clinical diagnosis and surveillance of IAVs, especially in the resource-limited settings. The proposed strategy establishes new avenues for developing a simple and rapid diagnostic system for viral infections and biomolecules. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Elsevier B.V. | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Label-free visual assayInfluenza A virusesPeptide nucleic acidGold nanoparticles | en_US |
dc.title | Label-free peptide nucleic acid biosensor for visual detection of multiple strains of influenza A virus suitable for field applications | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Article | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Analytica Chimica Acta | en_US |
dc.publication.volumeno | 1093 | en_US |
dc.publication.pagenumber | 123-130 | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | https://doi.org/10.1016/j.aca.2019.09.060 | en_US |
dc.publication.authorAffiliation | ICAR::National Institute of High Security Animal Diseases | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.naasrating | 11.98 | en_US |
Appears in Collections: | AS-NIHSAD-Publication |
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