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http://krishi.icar.gov.in/jspui/handle/123456789/2978| Title: | Cloning, characterization and expression of a new cry1Ab gene from DOR Bt-1, an indigenous isolate of Bacillus thuringiensis. |
| Other Titles: | Not Available |
| Authors: | V. Prathap Reddy N. Narasimha Rao P.S. Vimala Devi S. Sivaramakrishnan M. Lakshmi Narasu V. Dinesh Kumar |
| Published/ Complete Date: | 2013-01-01 |
| Project Code: | APNL and Institute project |
| Keywords: | Bacillus thuringiensis Bioassay Cloning Protein expression Cry1ab26 ELISA SDS-PAGE |
| Publisher: | Springer |
| Citation: | Reddy VP, Rao NN, Vimala Devi PS, Narasu ML, Sivaramakrishnan S and Dinesh Kumar V (2013) Cloning, characterization and expression of a new cry1Ab gene from DOR Bt-1, an indigenous isolate of Bacillus thuringiensis. Molecular Biotechnology, 54, Issue 3, pp 795-802 Published online first on December 9, 2012 DOI 10.1007/s12033-012-9627-3 |
| Series/Report no.: | Not Available; |
| Abstract/Description: | A new cry1Ab gene was cloned from the promising local isolate, DOR Bt-1, a Bacillus thuringiensis strain isolated from castor semilooper (Achaea janata L.) cadavers from castor bean (Ricinus communis L.) field. The nucleotide sequence of the cloned cry1Ab gene indicated that the open reading frame consisted of 3,465 bases encoding a protein of 1,155 amino acid residues with an estimated molecular weight of 130 kDa. Homology comparisons revealed that the deduced amino acid sequence of cry1Ab had a variation of seven amino acid residues compared to those of the known Cry1Ab proteins in the NCBI database and this gene has been designated as cry1Ab26 by the B. thuringiensis d-endotoxin Nomenclature Committee. cry1Ab26 was cloned into pET 29a(?) vector and expressed in E. coli strain BL21 (DE3) under the control of T7 promoter with IPTG induction. ELISA, SDS-PAGE, and Western blot analysis confirmed the expression of 130-kDa protein. Insect bioassays with neonate larvae of Helicoverpa armigera showed that the partially purified Cry1Ab26 caused 97 % mortality within 5 days of feeding |
| Description: | Not Available |
| ISSN: | ISSN: 1073-6085 (print version) ISSN: 1559-0305 (electronic version) |
| Type(s) of content: | Research Paper |
| Sponsors: | AP-Netherlands Biotechnology Scheme and Institute |
| Language: | English |
| Name of Journal: | Molecular Biotechnology |
| NAAS Rating: | 8.02 |
| Volume No.: | 54: |
| Page Number: | 795–802 |
| Name of the Division/Regional Station: | Crop Improvement |
| Source, DOI or any other URL: | DOI 10.1007/s12033-012-9627-3 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/2978 |
| Appears in Collections: | CS-IIOR-Publication |
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