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Title: | A simple method for screening gray mold of castor (Ricinus communis L) under artificial conditions |
Other Titles: | Screening method for gray mold of castor |
Authors: | Sujatha. T.Parvathy M.A. Raoof P.Jagadeesh Bewin Douglas |
Published/ Complete Date: | 2016-01-01 |
Project Code: | DOR 103-10 |
Keywords: | Amphobotryis ricini Botryotinia ricini castor gene expression gray mold sporulation susceptible |
Publisher: | Indian Journals |
Citation: | Sujatha. T. Parvathy,M.A. Raoof, P. Jagadesh and Bewin Douglas (2016). Applied Biological Research, 18 (2): 131-138. doi: 10.5958/0974-4517.2016.00021.5 |
Series/Report no.: | Not Available; |
Abstract/Description: | Botryotinia ricini (Godfrey) Whetzel, the gray mold pathogen of castor, inflicts serious crop losses in short periods. The present study was aimed at to develop a season-independent screening technique for gray mold in castor. Conidia from in vitro-raised pure culture of the anamorph Amphobotrys ricini were sprayed on separated mature as well as immature capsules of 4 castor genotypes (DCS 9, RG 2787, RG 3309 and RG 3216 R), placed in glass petriplates and incubated at 22–24ºC for 7 days. Genotype DCS-9 was highly susceptible to the disease, while genotype RG 3216 R exhibited least susceptibility. Immature capsules were more susceptible to disease than mature capsules. RNA degradation occurred in treated samples 2 days after treatment and control gene (UBQ) expression was unaffected till 4 hr post-inoculation. Thus a reproducible, lab-based, season-independent, screening technique using petri-plates and severed castor capsules was developed for differential gene expression analysis of candidate genes involved in early infection and elucidating host-pathogen interactions in castor gray mold. |
Description: | Institute project |
ISSN: | Not Available |
Type(s) of content: | Article |
Sponsors: | ICAR- IIOR |
Language: | English |
Name of Journal: | Applied Biological Research |
NAAS Rating: | 4.96 |
Volume No.: | 18 |
Page Number: | 131-138 |
Name of the Division/Regional Station: | Crop Improvement |
Source, DOI or any other URL: | DOI : 10.5958/0974-4517.2016.00021.5 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/3015 |
Appears in Collections: | CS-IIOR-Publication |
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