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http://krishi.icar.gov.in/jspui/handle/123456789/31762
Title: | Molecular and biochemical characterisation of polyphenol oxidases in developing kernels and senescing leaves of wheat (Triticum aestivum) |
Other Titles: | Not Available |
Authors: | Aravind K. Jukanti, Phil L. Bruckner and Andreas M. Fischer |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | Montana State University, Bozeman |
Published/ Complete Date: | 2006-06-01 |
Project Code: | Not Available |
Keywords: | enzyme activation, partial proteolysis, polyphenol oxidase, Triticum aestivum, wheat |
Publisher: | Not Available |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | Polyphenol oxidases (PPOs) have been implicated in plant defence reactions. From an applied point of view, high PPO activity is associated with browning / darkening of fresh and processed food. Owing to its complex genome and economic importance, wheat (Triticum aestivum L.) represents an interesting system to advance our understanding of plant PPO function. We have previously shown that wheat PPOs are organised in a multigene family, consisting of two distinct phylogenetic clusters with three members each. In this study, we demonstrate that members of one cluster are not expressed in developing kernels or senescing flag leaves. Transcriptional regulation of one major gene in the other cluster largely controls PPO levels in these tissues, at least in the wheat varieties used for this study. Our data further indicate that the product of this gene is present as a latent enzyme during early kernel development, and that the latent enzyme is activated during later developmental phases. Enzyme activation can be achieved in vitro by limited tryptic digestion, but our data do not indicate activation by a proteolytic mechanism in vivo. Together, results presented in this study provide important insights into the regulation ofwheat PPO function |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Functional Plant Biology |
NAAS Rating: | 8.62 |
Volume No.: | 33 |
Page Number: | 685–696 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/31762 |
Appears in Collections: | CS-IIRR-Publication |
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