. Development and characterization of low erucic /low glucosinolate lines in Indian mustard.

Abstract

The objective of this study was to develop low glucosinolate mustard (Brassica juncea Coss.). This was accompiished through an interspecific cross between an Indian type 3-butenyl glucosinolate containing B. junc€d selection and a "Bronowski-gene(s)" containing low glicosinolate B. campesiris L. followed by backcross_ing to the B. junceapatent. S;d oiBClF, plants, ancl selected plants of BC1F3 and BC,F.. generations were analyzed for glircosinolate content by gis chromatography. Total aliphatic glucosinolate contents of individual BC'F2 plants ranged from 57 to 204 pmol g ' meal' A single BC,F, plant grown from thebC,F, plani with the lowest glucosinolate^content, identified is 1058, was found to contain less than I pcmol g meal of^total aliphatic glucosinolates. The glucosinolate content in individual plants of the BCtFo generatton of plunt 1058 rangeJfrom 0.8 to 2.9 p.mol g I meal. Field grown progeny of 1058 at three locations in 1987 and at one location in 1988 in Saskatchewan contained less than iO p-of g I meal of total aliphatic glucosinoiates. Therefore, the low glucosinolate characteristic of selection 1058 can be considered genetically stable' Progeny of plant 1058 had plant morphology and seed coat reticulation of B. juncea, but poor fertility (< 5 seeds per pod). ThE-development of this low glucosinolate plant is an achievement that should aliow the breeding of canola quality oilseed B. juncea mustard.