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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/32681
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | ICAR_CRIDA | en_US |
dc.date.accessioned | 2020-02-25T06:53:03Z | - |
dc.date.available | 2020-02-25T06:53:03Z | - |
dc.date.issued | 2010 | - |
dc.identifier.citation | Not Available | en_US |
dc.identifier.issn | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/32681 | - |
dc.description | Not Available | en_US |
dc.description.abstract | An efficient protocol for shoot bud induction and proliferation employing half cotyledonary node with intact cotyledon explants derived from two-day-old seedlings of mung bean pre-conditioned on 6- benzylaminopurine (BAP) has been achieved. Explants were cultured for four weeks each on MS B5 + 12.5 μM BAP and MS B5 + 5 μM BAP +0.05 μM α- naphthaleneacetic acid (NAA ), respectively, as shoot bud induction and shoot elongation and proliferation media, gave the best regeneration response. The removal of the pre-existing buds from explants at 12 days in shoot bud induction medium led to enhanced regeneration response. Light microscopic observations on 14-day-old explants confirmed direct organogenesis route of regeneration. Elongated shoots (>2 cm) excised from the regenerating cultures were successfully rooted on half MS B5 medium containing 2.46 μM indolebutyric acid (IBA). About 90% of the rooted plantlets, efficiently hardened in pots having soil and farm yard manure, flowered and produced pods with viable seeds upon reaching maturity. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Yadav, S.K., Gopala Krishna, M., Maheswari, M. and Venkateswarlu, B. | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | BAP, half cotyledonary node with intact cotyledon, mung bean, regeneration | en_US |
dc.title | High frequency induction of multiple shoots and plant regeneration from cotyledonary node explant of mung bean (Vigna radiata L Wilczek) | en_US |
dc.title.alternative | High frequency induction of multiple shoots and plant regeneration from cotyledonary node explant of mung bean (Vigna radiata L Wilczek) | en_US |
dc.type | Technical Report | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Journal of Plant Biochemistry and Biotechnology | en_US |
dc.publication.volumeno | 19(2) | en_US |
dc.publication.pagenumber | 267-270 | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | Not Available | en_US |
dc.publication.authorAffiliation | ICAR_CRIDA | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.naasrating | 6.77 | en_US |
Appears in Collections: | NRM-CRIDA-Publication |
Files in This Item:
File | Description | Size | Format | |
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61 High frequency induction of multiple shoots and plant regeneration from cotyledonary node explant of mung bean.pdf | 146.8 kB | Adobe PDF | View/Open |
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