Superovulation and non-surgical embryo flushing in Indian camel (Camelus dromedarius)

Abstract

The embryo transfer technology which offers rapid exploitation of genetic potential of superior female has not been standardized in the Indian dromedary. Preliminary trials were conducted for developing embryo transfer technology in Indian camels. The donor she camels were examined by ultrasound for the presence of dominant follicle in ovaries. Various protocols were attempted for superovulation. The two-way long Foley’s catheter (26”, 18 Fr, 30 C>C.) was used for embryo flushing. The air bulb of catheter was fixed just inside the uterine body to flush both horns simultaneously. DPBS (1.5-2 litres) was used for flushing in about 12 to 14 releases. The media recovered was filtered through EmCon filter and filtrate was searched under stereozoom microscope for embryos. A total of seven embryos were collected out of eight superovulatory treatments. One good quality morula was transferred in recipient.