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http://krishi.icar.gov.in/jspui/handle/123456789/38287| Title: | Monitoring a biocontrol rhizobacterium Pseudomonas glumae using Tn5::lacZ marker. |
| Other Titles: | Not Available |
| Authors: | Pal, K. K. Tilak, K. V. B. R Saxena, A. K Rinku Dey Singh, C. S. |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | Directorate of Groundnut Research Division of Microbiology, Indian Agricultural Research Institute, New Delhi 110 012, India. |
| Published/ Complete Date: | 2000 |
| Project Code: | Not Available |
| Keywords: | Cotton Molecular marker Population dynamics Pseudomonas glumae, EM85 Tn5::lacZ |
| Publisher: | Association of Microbiologists of India |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Pseudomonas glumae [Burkholderia glumae] EM85 (Afu+Sid+Flu+HCN+), a potent biocontrol agent for phytopathogenic Rhizoctonia solani of cotton, was monitored using Tn5::lacZ molecular marker. The introduction of lacZ into the chromosome of EM 85 resulted in deficient and overproducing mutants of different antifungal traits. Fluorescent pigments and antifungal antibiotics were responsible for biocontrol of R. solani. Colonies of wild type, isogenic, deficient and overproducing mutants were selected using Lac+ phenotype, antibiotic resistance markers and fluorescence. A comparison of wild type and mutants in terms of their abilities to colonize the root zones of cotton revealed that in general, deficient mutants (Afu-, Sid-, Flu-, HCN- etc.) built up higher population than did the wild type, isogenic and overproducing mutants. This indicated that mutational inactivation or overproduction of antifungal traits did not affect the establishment, population dynamics and ecological fitness of P. glumae EM85. |
| Description: | Not Available |
| ISSN: | 0046-8991 |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Indian Journal of Microbiology |
| Volume No.: | 40 |
| Page Number: | 21-24 |
| Name of the Division/Regional Station: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/38287 |
| Appears in Collections: | CS-DGR-Publication |
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