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Title: | Anthocyanin profiling using UV-Vis spectroscopy and liquid chromatography mass spectrometry |
Other Titles: | Not Available |
Authors: | Saha S., Singh J., Paul A., Sarkar R., Khan Z., Banerjee Kaushik |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::National Research Centre for Grapes ICAR::Indian Agricultural Research Institute |
Published/ Complete Date: | 2020-01-29 |
Project Code: | Not Available |
Keywords: | Anthocyanin Profiling UV-Vis Spectroscopy Liquid Chromatography Mass Spectrometry |
Publisher: | Oxford University Press |
Citation: | Saha S., Singh J., Paul A., Sarkar R., Khan Z., Banerjee Kaushik (2019). Anthocyanin profiling using UV-Vis spectroscopy and liquid chromatography mass spectrometry. Journal of AOAC International 103(1): 23-39. DOI: https://doi.org/10.5740/jaoacint.19-0201 |
Series/Report no.: | Not Available; |
Abstract/Description: | Background: As a powerful antioxidant and natural colorant, anthocyanins are being used increasingly as a component of food supplements and nutraceutical products. Hence, its characterization is a prerequisite for further exploration of its nutraceutical potential. UV-Vis and MS are the two important techniques, which have been largely employed for the qualitative and quantitative determination of anthocyanins. However, a comprehensive review of the applications of these techniques in literature is scarce. Objective: This paper aims to review the utilization of UV-Vis spectral data as well as mass spectral data for characterization and putative identification of anthocyanins with approaches of quantification. Methods: The techniques described in literature have been thoroughly reviewed and comparatively evaluated. The complementary approaches of UV-Vis and MS spectra have been discussed for identification and quantification of these compounds. Results: Valuable information about the chemical composition and structure of anthocyanins can be predicted from the UV-Vis spectral data, such as number and type of glycosylation as well as absence or presence of acylation, to name a few. It is also pointed out that for their structural confirmation, selectivity of mass detectors with unit and high-resolution analysis could be effective. Conclusions: The combination of LC-MS with UV-Vis spectroscopy provides complementary information on structural details of anthocyanins. In case the analytical reference standards are available, a triple quadrupole mass spectrometer provides selectivity and quantitative sensitivity in analysis. On the other hand, high-resolution MS analysis provides valuable information for tentative identification during nontarget screening of compounds when the reference standard is not available. |
Description: | Not Available |
Type(s) of content: | Journal |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Journal of AOAC International |
Volume No.: | 103 |
Page Number: | 23-39 |
Name of the Division/Regional Station: | National Reference Laboratory |
Source, DOI or any other URL: | https://doi.org/10.5740/jaoacint.19-0201 |
URI: | https://doi.org/10.5740/jaoacint.19-0201 http://krishi.icar.gov.in/jspui/handle/123456789/38645 |
Appears in Collections: | HS-NRCG-Publication |
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