In vitro regeneration of Garcinia indica using leaf explants

Abstract

Regeneration of plantlets via adventitious bud differentiation on leaf explants of Garcinia indica (Thouars) Choisy, a medicinally important apomictic tropical tree species was done. Leaves were cultured on Murashige and Skoog's medium supplemented with cytokinins (6-Benzylaminopurine, BAP, kinetin and Thidiazuron, TDZ) alone and in combination with auxin (a-Napthaleneacetic acid, NAA) for direct shoot proliferation. Maximum number of shoots were formed in 63.89% explants on MS medium supplemented with 1.0 ,..,M TDZ after 2-4 weeks of culture. Elongation of the induced shoots was achieved on MS basal medium containing 0.2% activated charcoal. Maximum rooting (83.33%) was observed in shoots cultured on half-strength MS medium supplemented with 10 ,..,M mA. The plantlets were successfully transferred to soil after hardening.