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http://krishi.icar.gov.in/jspui/handle/123456789/39657
Title: | Induction of somatic embryogenesis in Pinus roxburghii Sarg |
Other Titles: | Not Available |
Authors: | S. Arya Rajwant K. Kalia I. D. Arya |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Central Arid Zone Research Institute |
Published/ Complete Date: | 2000-07-01 |
Project Code: | Not Available |
Keywords: | Conifers Pinusroxburghii Somatic embryogenesis Zygotic embryos Tissue culture |
Publisher: | Not Available |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | Embryogenic calli were initiated from embryonic explants of Pinus roxburghii using female gametophytes containing immature pre-cotyledonary embryos. Zygotic embryos were collected at different developmental stages and cultured on various media. Initiation of embryogenic calli was achieved in pre-cotyledonary zygotic embryos of a 0.1-mm to 1.2-mm embryonal head on Douglus fir cotyledon revised medium (DCR) medium supplemented with 2,4-D or NAA and BA. Embryogenic callus development was initiated from the suspensor region of immature embryos. The method of immature embryo culture was significant as rapid embryogenic callus development occurred in megagametophytes where the suspensor was stretched onto the medium from the cut micropylar end. Sixty embryogenic lines were established from 2500 explants cultured during one season. A pro-embryo with six to eight meristematic cells and suspensor of six to ten long, vacuolated cells dominated the early phase of the callus development. Cleavage polyembryony occurred in proliferating callus, constituting a method of multiplication of these somatic embryos. Somatic embryos developed to stage-I and stage-II embryos on DCR medium supplemented with 5 μM 2,4-D or 10 μM NAA. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Plant Cell Reports |
NAAS Rating: | 9.83 |
Volume No.: | 19 |
Page Number: | 775-780 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | https://link.springer.com/article/10.1007/s002990000197 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/39657 |
Appears in Collections: | NRM-CAZRI-Publication |
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