KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/4155
Title: | Retarded electrophoretic mobility of W chromosome specific DNA fragment from chicken |
Other Titles: | Not Available |
Authors: | SANTOSH HAUNSHI AND S.C. SAXENA |
Published/ Complete Date: | 2008-03-04 |
Project Code: | Not Available |
Keywords: | W chromosome, chicken, retarded mobility, agarose, PAGE |
Publisher: | Indian Poultry Science Association |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | In the study we report the unusually slow mobility of W chromosome specific DNA fragment. The primers designed to amplify a 481 bp DNA fragment of W chromosome of female chicken revealed its size on agarose gel electrophoresis to be 556 bp. The mobility of this fragment was still slower in polyacrylamide gel electrophoresis. This trend was observed in indigenous, exotic and Vanaraja varieties of chicken. This phenomenon of slow mobility of W chromosome specific DNA fragment was cross checked with the control DNA fragment of 256 bp. Delayed migration of PCR products was observed for other fragment of W chromosome repeat as well. Sequencing of PCR products revealed that the fragments were comprised of clusters of A (2-5) and T (2-5) sequences separated by (5-7) relatively G/C rich sequences. The anomalous behavior of slow migration of W chromosome specific fragments could be attributed to the occurrence of DNA curvatures because of the above mentioned sequence characteristics |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | SERC, Department of Science and Technology |
Language: | English |
Name of Journal: | Indian Journal of Poultry Science |
NAAS Rating: | 5.85 |
Volume No.: | 43 |
Page Number: | 32-34 |
Name of the Division/Regional Station: | Division of Poultry Science |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/4155 |
Appears in Collections: | AS-DPR-Publication |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
IJPS 2008.pdf | 537.99 kB | Adobe PDF | View/Open |
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.