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http://krishi.icar.gov.in/jspui/handle/123456789/42358| Title: | An insight into molecular interaction of PGIP with PG for banana cultivar |
| Other Titles: | Not Available |
| Authors: | Sunil Kumar Budheswar Dehury Gitanjali Tandon Sarika Jaiswal M. A. Iquebal Khurshid Ahmed Dipak T Nagrale Udai B Singh Yachana Jha Mahender Kumar Singh Arjun Singh Anil Rai B Paital Dinesh Kumar |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::National Bureau of Agriculturally Important Micro-organisms ICMR-Regional Medical Research Center, Bhubaneswar, India ICAR::Indian Agricultural Statistics Research Institute ICAR::Central Institute of Cotton Research N. V Patel College of Pure and Applied Sciences, V.V Nagar, Anand, Gujarat NBRC, Manesar, Gurgaon, India Redox Regulation Laboratory, Department of Zoology, Odisha University of Agriculture and Technology College of Basic Science and Humanities, Bhubaneswar, Odisha, India School of biosciences, IMS, Ghaziabad, India |
| Published/ Complete Date: | 2020-01-01 |
| Project Code: | Not Available |
| Keywords: | Host-Pathogen Interactions Molecular Modeling Pathogenesis-related Proteins |
| Publisher: | Not Available |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | PolyGalacturonase Inhibiting Proteins (PGIPs) are leucine rich repeat pathogenesis-related (PR) cell wall proteins, which interact and inhibit the PolyGalacturonase (PG), an enzyme secreted by the pathogen to degrade pectin. Interaction of PGIP with PG limits the vulnerability of PG by the activation of host defense response against pathogenic attack. Erwinia is gram-negative soft rot bacteria responsible for rhizome rot disease in banana and many other crop plants. The interaction of PG with PGIP is one of the crucial steps for plant-pathogen interaction. To study the molecular mechanism of PR proteins, we employed molecular modelling, protein-protein docking and molecular dynamics simulations of banana PGIP (bPGIP) with Erwinia carotovora PG (ecPG). Further, insilico site-directed mutagenesis was performed in Phaseolus vulgaris PGIP (pvPGIP2) to elucidate the interaction with ecPG. Docking and simulation studies divulge that binding of bPGIP and PvPGIP2 with active site residues of EcPG induces structural changes and thereby inhibit the enzyme. This study provides a unique insight into PG-PGIP interaction, which may help in the development of bacterial soft-rot resistant banana cultivars. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Article |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Frontiers in Bioscience-Landmark |
| NAAS Rating: | 8.75 |
| Volume No.: | 25 |
| Page Number: | 335-362 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | http://dx.doi.org/10.2741/4809 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/42358 |
| Appears in Collections: | AEdu-IASRI-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| An insight into molecular interaction of PGIP with PG for banana cultivar.pdf | 293.79 kB | Adobe PDF | View/Open |
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