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http://krishi.icar.gov.in/jspui/handle/123456789/4316| Title: | In vitro bud regeneration of Carthamus tinctorius and wild Carthamus species from leaf explants and axillary buds |
| Other Titles: | Not Available |
| Authors: | M. Sujatha V Dinesh Kumar |
| Published/ Complete Date: | 2007-01-01 |
| Project Code: | 101-2 |
| Keywords: | direct organogenesis, growth regulators, in vitro culture, tissue culture, wild safflowers |
| Publisher: | Springer nature |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | The organogenic competence of leaf explants of eleven Carthamus species including C. tinctorius on Murashige and Skoog (MS) medium supplemented with different concentrations of thidiazuron (TDZ) + α-naphthaleneacetic acid (NAA) and 6-benzyladenine (BA) + NAA was investigated. Highly prolific adventitious shoot regeneration was observed in C. tinctorius and C. arborescens on both growth regulator combinations and the shoot regeneration frequency was higher on medium supplemented with TDZ + NAA. Nodal culture of nine Carthamus species on media supplemented with BA and kinetin (KIN) individually revealed the superiority of media supplemented with BA over that of KIN in facilitating a higher shoot proliferation index. Proliferating shoots from axillary buds and leaf explants were transferred to medium supplemented with 1.0 mg dm-3 KIN or 0.5 mg dm-3 BA for shoot elongation. Elongated shoots were rooted on half-strength MS medium supplemented with 1.0 mg dm-3 each of indole-butyric acid (IBA) and phloroglucinol. The plantlets thus obtained were hardened and transferred to soil. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Biologia Plantarum |
| NAAS Rating: | 7.6 |
| Volume No.: | 51 |
| Page Number: | 782-786 |
| Name of the Division/Regional Station: | Crop Improvement |
| Source, DOI or any other URL: | https://link.springer.com/article/10.1007/s10535-007-0160-3 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/4316 |
| Appears in Collections: | CS-IIOR-Publication |
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