Combined effect of osmotic stress induced by polyethylene glycol-6000 and alcohol on in vitro growth of Vigna aconitifolia

Abstract

The present study wass conducted to determine the reliability of in vitro screening method. In vitro screening method proves to be an ideal method for screening large set of germplasm with less efforts accurately and cost effective. A set of 12 genotypes of moth bean were evaluated for desiccation tolerance at seedling stage against two chemical (PEG and alcohol) to check their response. Differential response was observed at 15% PEG and 1% ethanol on seedling level respectively. The twelve varieties were classified as tolerant, moderatly tolerant and sensitive based on number of seedlings showing wilting symptoms at seedling stage at 15% PEG and 1% alcohol. Variety RMO-435, RMM-12-Single, RMM-12-Poly and CZM-105 were considered tolerant showing 14 to 19% wilted seedlings. Varieties RMM-12-Zero, RMM-12-Double, RM0-423, CZM-18 were considered moderate tolerant with 19 to 42% wilted seedlings. Varieties showing more than 72% wilted seedlings (RMO-225, RM0-40, RMO-257, and CZM-04-01) were considered sensitive.