KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/43603
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sushil Kumar | en_US |
dc.contributor.author | Adinath S Palve | en_US |
dc.contributor.author | Swati K Patel | en_US |
dc.contributor.author | Sivasubramani Selvanayagam | en_US |
dc.contributor.author | Ramavtar Sharma | en_US |
dc.contributor.author | Abhishek Rathore | en_US |
dc.date.accessioned | 2020-12-23T11:15:44Z | - |
dc.date.available | 2020-12-23T11:15:44Z | - |
dc.date.issued | 1001-01-01 | - |
dc.identifier.citation | Kumar Sushil, Adinath S Palve, Swati K Patel, Sivasubramani Selvanayagamb,Ramavtar Sharma, Abhishek Rathore. 2020. Development of genomic microsatellite markers in cluster bean using next-generation DNA sequencing and their utility in diversity analysis. Current Plant Biology. 21 | en_US |
dc.identifier.issn | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/43603 | - |
dc.description | Not Available | en_US |
dc.description.abstract | With high global demand, seeds and gum of cluster bean are an essential raw material for various industries. Worldwide the low yield and productivity of guar (Cyamopsis tetragonoloba) create a large gap between demand and supply of guar. Therefore, to upsurge guar production, there is a need to improve the guar at genetic level. The genetic improvement of cluster bean is slower due to insufficient genomic resources, co-dominant marker system especially simple sequence repeats (SSRs) and inadequate information on the variability of germplasm. For development of microsatellite markers in cluster bean, DNA survey sequencing was carried out using the Miseq NGS system. DNA sequencing generated 73,934 sequences which harboured microsatellite repeat sequences. Successfully, a total of 15,399 marker-pairs were designed. The amplicon size ranged between 101–385 base-pairs. To validate the primers and to analyse the diversity in released varieties and germplasm of cluster bean, successfully amplifiable 21 primers pairs were used. With a mean of 2.05 alleles per primers, 21 bands were detected. The polymorphism information content (PIC) for polymorphic markers fluctuated between 0.04 0.67 (mean = 0.3). With the average dissimilarity coefficient of 0.11, four clusters were noticed with NJ analysis. The grouping pattern of genotypes indicated a low genetic variability in the guar gene pool. The results of this study suggested that instead of re-circulation of parental lines, hybrid breeding using diverse parents followed by selection should be performed for genetic improvement of cluster bean. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Not Available | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Cluster bean | en_US |
dc.subject | Diversity | en_US |
dc.subject | DNA marker | en_US |
dc.subject | NGS | en_US |
dc.subject | SSR | en_US |
dc.title | Development of genomic microsatellite markers in cluster bean using next generation DNA sequencing and their utility in diversity analysis | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Current Plant Biology | en_US |
dc.publication.volumeno | 21 | en_US |
dc.publication.pagenumber | Not Available | en_US |
dc.publication.divisionUnit | Div.III (PI&PM) | en_US |
dc.publication.sourceUrl | https://doi.org/10.1016/j.cpb.2019.100134 | en_US |
dc.publication.sourceUrl | https://www.sciencedirect.com/science/article/pii/S2214662819302130 | en_US |
dc.publication.authorAffiliation | ICAR::Central Arid Zone Research Institute | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
Appears in Collections: | NRM-CAZRI-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.