KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/43778
Title: | Potential of a rhizobacterium Bacillus subtilis (Bbv 57) on Fusarium oxysporum f. sp. gerberae and Meloidogyne incognita infecting Gerbera grown in protected cultivation. |
Authors: | Ramyabharathi, SA Sankari Meena, K. Rajendran, L. Raguchander, T. Jonathan,E.I. |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Indian Institute of Oilseeds Research |
Published/ Complete Date: | 2020-08-25 |
Project Code: | Not Available |
Keywords: | Bacillus subtilis, Fusarium oxysporum, Meloidogyne incognita |
Publisher: | Springer, Netherlands |
Citation: | Ramyabharathi,SA., Sankari Meena, K., Rajendran, L., Raguchander, T. and Jonathan,E.I. 2020. Potential of a rhizobacterium Bacillus subtilis (Bbv 57) on Fusarium oxysporum f. sp. gerberae and Meloidogyne incognita infecting Gerbera grown in protected cultivation. European Journal of Plant Pathology 158, 615–632. |
Series/Report no.: | Not Available; |
Abstract/Description: | Ninety five bacterial strains were isolated from the rhizosphere and soils of flower crops, specifically gerbera, carnation and tuberose, were preliminarily screened against Fusarium oxysporum f. sp. gerberae in vitro along with five elite strains Bbv 57, EPCO 16, EPC 5, EPC 8 and Pf 1 obtained from Department of Plant Pathology, TNAU, Coimbatore, India. The results revealed that the strain Bbv 57 had highest inhibition of 50.00% and showed the maximum value for the assays that is siderophore, hydrogen cyanide, IAA, GA3 and salicylic acid production (3.68 O.D. value; 14.05 μgml-1; 44.40 μg ml-1; 25.28 μg ml-1 and 19.25 μg ml-1 respectively). It also showed resistance to antibiotics namely ampicillin, erythromycin, clindamycin. The highest exopolysaccharides, biofilm production was observed and had lowest protease production clearly indicated that it is non - pathogenic to plants. Further, the polymerase chain reaction using16S rRNA intervening sequencing showed 100% homology to Bacillus subtilis (KF718836) and showed positive for quorum sensing regulator genes of aiiA and comQ. Additionally, the culture filtrate assay also produced significant reduction in egg hatching capacity and juvenile mortality of root-knot nematode Meloidogyne incognita. Further, the HPLC study showed 91.69 μg μl-1 of surfactin with the retention time of 2.304 min and 0.453 μg μl-1 of Iturin with the retention time of 8.739 min at 205 nm. Whereas, GCMS analysis has detected the aliphatic hydrocarbons responsible for antifungal, antibacterial and nematicidal activity. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Article |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | European Journal of Plant Pathology |
NAAS Rating: | 7.58 |
Volume No.: | 158 |
Page Number: | 615 – 632 |
Name of the Division/Regional Station: | Crop Protection |
Source, DOI or any other URL: | doi.org/10.1007/s10658-020-02087-6 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/43778 |
Appears in Collections: | CS-IIOR-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.