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http://krishi.icar.gov.in/jspui/handle/123456789/44214| Title: | Transformation of mothbean (Vigna aconotifolia) cotyledonary nodes through vacuum infiltration of Agrobacterium tumefaciens |
| Other Titles: | Not Available |
| Authors: | Kumar S Singh G Jat RS Saxena SN Sharma R |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Central Arid Zone Research Institute |
| Published/ Complete Date: | 2010-12-01 |
| Project Code: | Not Available |
| Keywords: | Agrobacterium tumefaciens cotyledonary nodes mothbean vacuum infiltration Vigna aconitifolia |
| Publisher: | Kumar S, Singh G, Jat RS, Saxena SN and Sharma R. 2010 |
| Citation: | Kumar S, Singh G, Jat RS, Saxena SN and Sharma R. 2010. Transformation of mothbean (Vigna aconotifolia) cotyledonary nodes through vacuum infiltration of Agrobacterium tumefaciens. Green Farming Vol. 1(6) : 551-554. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | The present investigation generates a rapid and convenient stable expression protocol based on vacuum infiltration of Agrobacterium tumefaciens cells into moth bean cotyledonary nodes. Vacuum infiltrated tissues showed transient GUS expression than did non infiltrated tissues. The leaflets arising from the transformed shoots on kanamycin supplemented media were found GUS positive during histochemical assay. We believe this to be the first report of this technique for putative gene expression in mothbean cotyledonary nodes. Mothbean cotyledonary nodes are one of the most regenerative tissues in legumes. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Green Farming |
| NAAS Rating: | 3.85 |
| Volume No.: | 1(6) |
| Page Number: | 551-554 |
| Name of the Division/Regional Station: | Div.III (PI&PM) |
| Source, DOI or any other URL: | ISSN 0974-0775 https://www.greenfarming.in/?articles=transformation-of-mothbean-vigna-aconitifolia-cotyledonary-nodes-through-vacuum-infiltration-of-agrobacterium-tumefaciens |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/44214 |
| Appears in Collections: | NRM-CAZRI-Publication |
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