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(An Institutional Publication and Data Inventory Repository)


  1. KRISHI Publication and Data Inventory Repository
  2. Crop Science A5
  3. ICAR-Indian Institute of Maize Research G3
  4. CS-IIMR-Publication
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"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item: http://krishi.icar.gov.in/jspui/handle/123456789/44909
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dc.contributor.authorAnil Kumaren_US
dc.contributor.authorChetana Aggarwalen_US
dc.contributor.authorKrishan Kumaren_US
dc.contributor.authorIshwar Singhen_US
dc.contributor.authorPranjal Yadavaen_US
dc.date.accessioned2021-01-20T10:59:25Z-
dc.date.available2021-01-20T10:59:25Z-
dc.date.issued2020-02-10-
dc.identifier.citationNot Availableen_US
dc.identifier.issnNot Available-
dc.identifier.urihttp://krishi.icar.gov.in/jspui/handle/123456789/44909-
dc.descriptionIn: abstract book of National Seminar on “Maize for Crop Diversification under Changing Climatic Scenario” jointly organized by MTAI, IIMR and PAU held at Ludhiana, February 9-10, 2020.en_US
dc.description.abstractThe post green revolution agriculture is based on generous application of nitrogen (N)-based fertilizers and high-yielding genotypes. Generally, plants cannot utilize more than 40% of the applied nitrogenous fertilizer; hence more than half of the applied fertilizer is lost to the environment and results in environmental pollution via acidification, eutrophication, and depletion of ozone layer by emission of greenhouse gas. Therefore, genetic improvement in nitrogen use efficiency (NUE) in crops is desirable for a sustainable and profitable agriculture. There is a need to identify key regulatory factors playing pivotal role in acquisition, transportation and utilization of N in plants. Among other factors, microRNA (miRNA) mediated gene regulation plays a crucial role in controlling low N stress adaptation and tolerance in plants. In this endeavor, the present study was undertaken to identify N stress responsive miRNAs in maize in tropical maize using high-throughput sequencing. The HKI-163 maize inbred line was grown hydroponically with sufficient nitrogen (2mM) and without nitrogen for 21 days. Observations were recorded on all important shoot and root physiological parameters. The root and shoot samples were deep sequenced for miRNA study. The expression analysis revealed 23 known miRNAs (11 up & 12 down-regulated) in leaf and 3 known miRNAs (1 up & 2 down-regulated) in root, which expressed differentially under N stress. We also identified 53 (20 up & 33 down-regulated) and 26 (9 up & 17 down-regulated) novel miRNAs in leaf and roots respectively. The knowledge gained will help understand the important roles that miRNAs play in maize, while responding to a nitrogen limiting environment.en_US
dc.description.sponsorshipNot Availableen_US
dc.language.isoEnglishen_US
dc.publisherNot Availableen_US
dc.relation.ispartofseriesNot Available;-
dc.subjectMaizeen_US
dc.subjectNitrogen fertilizeren_US
dc.subjectNitrogen use efficiency (NUE)en_US
dc.subjectmicroRNA profilingen_US
dc.titleProfiling of low-nitrogen stress responsive miRNAs in maize using high-throughput sequencingen_US
dc.title.alternativeNot Availableen_US
dc.typeOtheren_US
dc.publication.projectcodeNot Availableen_US
dc.publication.journalnameNot Availableen_US
dc.publication.volumenoNot Availableen_US
dc.publication.pagenumber184en_US
dc.publication.divisionUnitNot Availableen_US
dc.publication.sourceUrlNot Availableen_US
dc.publication.authorAffiliationICAR-Indian Institute of Maize Researchen_US
dc.ICARdataUseLicencehttp://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdfen_US
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