KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/45036
Title: | Introgression of crtRB1 through marker assisted backcross breeding (MABB) |
Other Titles: | Not Available |
Authors: | Yadav HK Gangoliya SS Yathish KR Kumar A Preeti Goshal T Srivastava S Kapoor U Kumar S Kumar R Singh SB Sekhar JC Das AK Neelam S Yadav OP Mahajan V Karjagi CG |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR-Indian Institute of Maize Research |
Published/ Complete Date: | 2020-02-09 |
Project Code: | Not Available |
Keywords: | Provitamin A |
Publisher: | Not Available |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | Maize (Zea mays L.) is grown on nearly 190 million ha in about 165 countries with total production yielding approximately 39% of total grain production. In India maize is third most important cereal crop after rice and wheat and accounts for around 10% of total food grain production. Maize contributes 25 to 35 per cent of total calories of the diets in several African, Asian and Latin American countries. Thus it is a valuable source of food and feed worldwide. Further enhancement of nutritional value through micronutrients like provitamin A in maize through biofortification could play an vital role in reducing the micronutrient malnutrition, the major challenge to ensure nutritional security in developing countries. Biofortification is the process by which the nutrient density of food crops is increased through conventional plant breeding and/or improved agronomic practices and/or modern biotechnology without sacrificing any characteristic that is preferred by consumers or most importantly to farmers. In the present study, gene encoding β-carotene hydroxylase 1 (crtRB1) alleles associated with higher β-carotene concentrations was introgressed into elite inbred line LM 17 and 19 using marker-assisted backcross breeding (MABB). The donor for crtRB1 gene was CIMMYT5 inbred line. For foreground selection crtRB1 gene-based simple sequence repeat (SSR) markers were successfully used in foreground selection. Presently introgressed lines are in BC2F2 generation. In LM17 × CIMMYT5 cross, out of 90 plants, 31 plants were homozygous, 42 plants were heterozygous while 17 plants were negative and in LM19 × CIMMYT5 cross out of 167 plants, 54 plants were homozygous, 87 plants were heterozygous while 26 plants were negative. The plants carrying gene of interest, were screened with SSR markers and recovery of recurrent parent genome (RPG) in background selection ranged between 82.40 - 93.70% for the LM17 × CIMMYT5 and 84.7091.30% for LM19 × CIMMYT5. The newly developed provitamin A near siogenic lines (NILs) developed in both the crosses are comparable with their recurrent parents for agronomic performance for different traits like days of anthesis, ear diameter, ear height, ear length, kernels per row and kernels rows, however the biochemical analysis is being carried out. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Proceedings |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Not Available |
Volume No.: | Not Available |
Page Number: | Not Available |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/45036 |
Appears in Collections: | CS-IIMR-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.