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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/45143
Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Vineet Kumar, Anita Rani, Reena Rawal | en_US |
dc.date.accessioned | 2021-01-30T10:11:05Z | - |
dc.date.available | 2021-01-30T10:11:05Z | - |
dc.date.issued | 2014-10-01 | - |
dc.identifier.citation | Not Available | en_US |
dc.identifier.issn | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/45143 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Lipoxygenase-2, controlled by single dominant gene, is prime cause of off-flavour generated in soy products. The objective of the present investigation was to validate the SSR markers (Sat_074 & Satt522) reported to be linked to Lox2 locus and to identify tightly linked SSR markers in F2 mapping population 1 and population 2, generated from crosses between Indian soybean varieties JS97-52 and JS93-05 as the conventional parents (Lox2Lox2) and PI596540 as the source of null allele of lipoxygenase-2 (lox2lox2), respectively. Parental polymorphism was surveyed using SSR markers Sat_074, Satt522 and 7 SSR markers in the neighbouring genomic region of Lox2 locus. F2:3 seeds from each F2 individual in both the mapping populations were phenotyped for the presence/absence of lipoxygenase-2 isozyme. The SSR marker Sat_074 was not found to be polymorphic for the parental combination JS97-52 x PI596540. Satt522 was found to be at 9.3 and 13.5 cM from Lox2 locus in mapping population 1 and 2, respectively. We found two additional new SSR markers Sat_417 and Satt656 closely linked to Lox2 locus. SSR marker Sat_417 was found to be at a close distance of 6.9 and 6.6 cM from Lox2 locus; while Satt656 was identified to be tightly linked to Lox2 locus at a distance of 2.7 and 2.1 cM in the mapping population 1 and 2, respectively. Thus, the SSR marker Satt656 can be employed in marker assisted selection for transferring null allele (lox2) of the lipoxygenase-2 in the background of popular Indian soybean cultivars. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | NISCAIR-CSIR, India | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Lipoxygenase 2, Lox 2 locus, off-flavour, soybean, SSR markers | en_US |
dc.title | Identification of simple sequence repeat markers tightly linked to lipoxygenase-2 gene in soybean. | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Indian Journal of Biotechnology | en_US |
dc.publication.volumeno | 13(4) | en_US |
dc.publication.pagenumber | 455-458 | en_US |
dc.publication.sourceUrl | Not Available | en_US |
dc.publication.authorAffiliation | Directorate of Soybean Research | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.naasrating | 6.41 | en_US |
Appears in Collections: | CS-DSBR-Publication |
Files in This Item:
File | Description | Size | Format | |
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IJBT 13(4) 455-458.pdf | 107.22 kB | Adobe PDF | View/Open |
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