KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/4561
Title: | RT LAMP assay: an alternative approach for profiling of bovine heat shock protein 70 gene in PBMC cultured model |
Other Titles: | Not Available |
Authors: | Sengar G S, Deb R*, Raja T V, Singh U, Kant R , Bhanuprakash V., Alyethodi R R, Kumar S, Verma P , Chakraborty S, Alex R, Singh R |
Published/ Complete Date: | 2017-05-27 |
Project Code: | AGB/Ext-DST/OXX03250/RD/(20/11/2015-19/11/2018) |
Keywords: | Absorbance Hsp70 RT-LAMP RT-PCR |
Publisher: | Springer |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | The purpose of this study is to develop a novel Reverse Transcriptase Loop-mediated isothermal amplification (RT-LAMP) based assay for in vitro profiling of heat shock protein 70 (Hsp70) in bovine peripheral blood mononuclear cell (PBMC) culture model utilizing the absorbance level of magnesium pyrophosphate—a by-product of LAMP reaction. A set of bovine Hsp70 specific RT-LAMP primers were designed to detect the differential absorbance level of magnesium pyrophosphate by-product which signifies the degree of Hsp70 amplification from cDNA of thermally induced cultured cells at different recovery periods. The study revealed significant (P < 0.05) correlation between absorbance level and the fold change of Hsp70 transcripts at different kinetic intervals of heat stress recovery in bovine PBMC cell culture models. RT-LAMP based absorbance assay can be used as an indicator to measure the degree of bovine Hsp70 transcripts produced during thermal stress and can be used as an alternative to the traditional Real time PCR assay. Developed RT-LAMP assay can be used as a cost-effective method for profiling of bovine HSP70 gene. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Science and Engineering Research Board, Government of India (YSS/2014/000279) |
Language: | English |
Name of Journal: | Molecular Biology Reports |
NAAS Rating: | 7.4 |
Volume No.: | Not Available |
Page Number: | Not Available |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | DOI 10.1007/s11033-017-4108-1 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/4561 |
Appears in Collections: | AS-CIRC-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.