KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/46902
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee J, Das Avijit, Yamaguchi M, Hashimoto J, Tsutsumi N, Uchimiya H and Umeda M | en_US |
dc.date.accessioned | 2021-04-30T06:01:35Z | - |
dc.date.available | 2021-04-30T06:01:35Z | - |
dc.date.issued | 2003-01-01 | - |
dc.identifier.citation | Not Available | en_US |
dc.identifier.issn | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/46902 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Cyclin‐dependent kinases (CDKs) are involved in the control of cell cycle progression. Plant A‐type CDKs are functional homologs of yeast Cdc2/Cdc28 and are expressed throughout the cell cycle. In contrast, B‐type CDK (CDKB) is a family of mitotic CDKs expressed during the S/M phase, and its precise function remains unknown. Here, we identified two B2‐type cyclins, CycB2;1 and CycB2;2, as a specific partner of rice CDKB2;1. The CDKB2;1–CycB2 complexes produced in insect cells showed a significant level of kinase activity in vitro, suggesting that CycB2 binds to and activates CDKB2. We then expressed green fluorescent protein (GFP)‐fused CDKB2;1 and CycB2;2 in tobacco BY2 cells to investigate their subcellular localization during mitosis. Surprisingly, the fluorescence signal of CDKB2;1‐GFP was tightly associated with chromosome alignment as well as with spindle structure during the metaphase. During the telophase, the signal was localized to the spindle midzone and the separating sister chromosomes, and then to the phragmoplast. On the other hand, the CycB2;2‐GFP fluorescence signal was detected in nuclei during the interphase and prophase, moved to the metaphase chromosomes, and then disappeared completely after the cells passed through the metaphase. Co‐localization of CDKB2;1‐GFP and CycB2;2‐GFP on chromosomes aligned at the center of the metaphase cells suggests that the CDKB2–CycB2 complex may function in retaining chromosomes at the metaphase plate. Overexpression of CycB2;2 in rice plants resulted in acceleration of root growth without any increase in cell size, indicating that CycB2;2 promoted cell division probably through association with CDKB2 in the root meristem. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Wiley-Blackwell | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Cell cycle, cell division, cyclin, cyclin dependent kinase, mitosis, rice | en_US |
dc.title | Cell cycle function of a rice B2-type cyclin interacting with a B-type cyclin-dependent kinasse | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Article | en_US |
dc.type | Journal | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | The Plant Journal | en_US |
dc.publication.volumeno | 34 | en_US |
dc.publication.pagenumber | 417 – 425 | en_US |
dc.publication.divisionUnit | Institute of Molecular and Cellular Biosciences | en_US |
dc.publication.sourceUrl | https://doi.org/10.1046/j.1365-313X.2003.01736.x | en_US |
dc.publication.authorAffiliation | The University of Tokyo | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.naasrating | 12.14 | en_US |
dc.publication.naasrating | Not Available | - |
dc.publication.impactfactor | 6.141 | en_US |
Appears in Collections: | AEng-NINFET-Publication |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Cyclin paper.pdf | 235.32 kB | Adobe PDF | View/Open |
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.