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http://krishi.icar.gov.in/jspui/handle/123456789/47020
Title: | EvaGreen-based Multiplex Real-time PCR Assay for Rapid Differentiation of Wild-Type and Glycoprotein E-Deleted Bovine Herpesvirus-1 Strains |
Authors: | Sachinkumar Shivajirao Pawar |
Published/ Complete Date: | 2017-10-1 |
Keywords: | EvaGreen,Multiplex,Differentiation,Wild-Type,Bovine Herpesvirus-1 |
Publisher: | Not Available |
Citation: | Pawar SS, Meshram CD, Singh NK, Saini M, Mishra BP, Gupta PK. EvaGreen-based Multiplex Real-time PCR Assay for Rapid Differentiation of Wild-Type and Glycoprotein E-Deleted Bovine Herpesvirus-1 Strains. Anim Biotechnol. 2017 Oct 2;28(4):248-252. doi: 10.1080/10495398.2016.1268620. Epub 2017 Jan 6. P |
Abstract/Description: | Bovine herpesvirus-1 (BoHV-1) is an important viral pathogen causing significant economic losses to the cattle industry. Glycoprotein E-deleted marker vaccines form the basis for BoHV-1 control programs widely, wherein detection and differentiation of wild-type and gE-deleted vaccine strains is of crucial importance for proper disease management. In the present study, we report an EvaGreen-based multiplex real-time polymerase chain reaction (EGRT-PCR) assay for rapid differentiation of wild-type and glycoprotein E-deleted strains of BoHV-1. The EGRT-PCR assay could simultaneously detect two viral genes (glycoprotein B and E) and an internal positive control gene (bovine growth hormone- bGH), in a single-tube reaction. The analytical sensitivity of the EGRT-PCR assay was as little as 10 copies of the BoHV-1 DNA per reaction. The modified real-time PCR assay could successfully differentiate wild-type and gE-deleted BoHV-1 strains based on gene specific melting temperatures (Tm) peaks. Our results have shown that the EGRT-PCR developed in this study might prove to be a promising tool in disease management by enabling rapid differentiation of wild-type and gE-deleted strains of BoHV-1. |
Description: | Not Available |
ISBN: | Not Available |
ISSN: | 1049-5398 |
Type(s) of content: | Research Paper |
Language: | English |
Name of Journal: | Animal Biotechnology |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/47020 |
Appears in Collections: | AS-IVRI-Publication |
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