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http://krishi.icar.gov.in/jspui/handle/123456789/4754
Title: | DNA bar-Coding of Indian coldwater fishes of genus Schizothorax (Family: Cyprinidae) from western Himalaya |
Other Titles: | Not Available |
Authors: | Suresh Chandra Ashoktaru Barat Mahender Singh Birender Kumar Singh Rakesh Matura |
Published/ Complete Date: | 2012 |
Project Code: | Not Available |
Keywords: | Cytochrome Oxidase Subunit I % Bar-coding % Fish % MtDNA % Schizothorax |
Publisher: | IDOSI Publications |
Citation: | Chandra, S., Barat, A., Singh, M., Singh, B.K. and Matura, R., 2012. DNA bar-coding of Indian coldwater fishes of genus Schizothorax (family: Cyprinidae) from Western Himalaya. World Journal of Fish and Marine Sciences, 4(4), pp.430-435. |
Series/Report no.: | Not Available; |
Abstract/Description: | The rapid and accurate characterization of species using morphological data is a critical constraint. To overcome this, species identification using molecular tools has been supplemented in many studies in present era. The present study was designed to test the utility of Cytochrome Oxidase I (COI) DNA barcodes for the identification of two commercially important coldwater species of Genus Schizothorax (Snow trout), Family Cyprinidae, from Uttarakhand Himalayas. COI gene (655 bp) was amplified using PCR and sequenced. The mean intra-specific nucleotide sequence divergence was 1.75% (range 0.00-3.50%) and inter-specific divergence of S. richardsonii is 0.00% (range 0.00040-0.00080%) and S. progastus is 0.00% (range 0.00036- 0.000206), respectively. Although, DNA bar-coding aims to develop species identification systems, some phylogenetic signal was apparent in the data. It was concluded that COI sequencing or ‘bar-coding’, was found to be suitable for the identification of coldwater fish species. |
Description: | Not Available |
ISSN: | 2078-4589 |
Type(s) of content: | Research Paper |
Sponsors: | Indian Council of Agriculture Research (ICAR) and Department of Biotechnology (DBT), New Delhi |
Language: | English |
Name of Journal: | World Journal of Fish and Marine Sciences |
Volume No.: | 4 (4) |
Page Number: | 430-435 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/4754 |
Appears in Collections: | FS-NBFGR-Publication |
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36375-93202.pdf | 393.17 kB | Adobe PDF | View/Open |
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