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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/47610
Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Joshi I, Kumar A, Singh AK, Kohli D, Raman KV, Sirohi A, Chaudhury A and Jain PK | en_US |
dc.date.accessioned | 2021-07-09T11:58:23Z | - |
dc.date.available | 2021-07-09T11:58:23Z | - |
dc.date.issued | 2019-11-01 | - |
dc.identifier.citation | Not Available | en_US |
dc.identifier.issn | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/47610 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Root-knot nematodes (RKNs) are devastating parasites that infect thousands of plants. As RKN infection is facilitated by oesophageal gland effector genes, one such effector gene, Mi-msp2, was selected for a detailed characterization. Based on domain analysis, the Mi-MSP2 protein contains an ShKT domain, which is likely involved in blocking K+ channels and may help in evading the plant defence response. Expression of the Mi-msp2 gene was higher in juveniles (parasitic stage of RKNs) than in eggs and adults. Stable homozygous transgenic Arabidopsis lines expressing Mi-msp2 dsRNA were generated, and the numbers of galls, females and egg masses were reduced by 52–54%, 60–66% and 84–95%, respectively, in two independent RNAi lines compared with control plants. Furthermore, expression analysis revealed a significant reduction in Mi-msp2 mRNA abundance (up to 88%) in female nematodes feeding on transgenic plants expressing dsRNA, and northern blot analysis confirmed expression of the Mi-msp2 siRNA in the transgenic plants. Interestingly, a significant reduction in the reproduction factor was observed (nearly 40-fold). These data suggest that the Mi-msp2 gene can be used as a potential target for RKN management in crops of economic importance. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Nature Scientific Reports | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | nematode resistance , RNAi, gene | en_US |
dc.title | Development of nematode resistance in Arabidopsis by HD-RNAi-mediated silencing of the effector gene Mi-msp2. | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Not Available | en_US |
dc.publication.volumeno | Not Available | en_US |
dc.publication.pagenumber | Not Available | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | https://doi.org/10.1038/s41598-019-53485-8 | en_US |
dc.publication.authorAffiliation | ICAR::National Institute for Plant Biotechnology | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.impactfactor | 4.0 | en_US |
Appears in Collections: | CS-NIPB-Publication |
Files in This Item:
File | Description | Size | Format | |
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PKJ scientific reports final published article nov 2019.pdf | 1.91 MB | Adobe PDF | View/Open |
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