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http://krishi.icar.gov.in/jspui/handle/123456789/48568| Title: | Simple multiplex PCR for rapid diagnosis of sex of ducks and duck embryos |
| Other Titles: | Not available |
| Authors: | Santosh Haunshi, Saxena, S. C. Pattanayak, A. Bandyopadhaya, S. Tyagi, A.K. Bujarbaruah, K. M. |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Research Complex for NEH Region ICAR::Directorate of Poultry Research |
| Published/ Complete Date: | 2008-06-01 |
| Project Code: | Not Available |
| Keywords: | Multiplex PCR, duck, embryo, sex diagnosis |
| Publisher: | Taylor and Francis |
| Citation: | Haunshi, S., S. C. Saxena, A. Pattanayak, S. Bandyopadhaya, A. K. Tyagi and K. M. Bujarbaruah. 2008. Simple Multiplex PCR for Rapid Diagnosis of Sex of Ducks and Duck Embryos, Journal of Applied Animal Research, 33:2, 117-120, DOI: 10.1080/09712119.2008.9706911 |
| Series/Report no.: | Not Available; |
| Abstract/Description: | A simple and rapid multiplex polymerase chain reaction (PCR) was developed for quick diagnosis of sex of duck and duck embryos. W chromosome specific DNA sequence was selected and primers were designed to amplify 335 bp fragment from female sex while 16s ribosomal sequence was selected to design primers to amplify 468 bp PCR products both in male and female sex as an internal control. Nucleotide sequences of W chromosome specific DNA fragments of Khaki Campbell and Indian Runner breeds of duck were found to be identical both in size and sequences, The study concluded that the protocol was successful in precisely identifying the gender of ducklings belonging to both Indian Runner and Khaki Campbell breeds of ducks and duck embryos |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | SERC |
| Language: | English |
| Name of Journal: | Journal of Applied Animal Research |
| Journal Type: | Research Journal |
| NAAS Rating: | 7.25 |
| Impact Factor: | 1.630 |
| Volume No.: | 33(2) |
| Page Number: | 117-120 |
| Name of the Division/Regional Station: | Poultry Science |
| Source, DOI or any other URL: | https://doi.org/10.1080/09712119.2008.9706911 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/48568 |
| Appears in Collections: | AS-DPR-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| JAAR 2008 Duck sexing.pdf | 123.2 kB | Adobe PDF | View/Open |
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