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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/4881
Full metadata record
DC Field | Value | Language |
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dc.contributor.author | N. Sood | en_US |
dc.contributor.author | D.K. Chaudhary | en_US |
dc.contributor.author | A. Singh | en_US |
dc.contributor.author | G. Rathore | en_US |
dc.date.accessioned | 2017-07-21T05:28:33Z | - |
dc.date.available | 2017-07-21T05:28:33Z | - |
dc.date.issued | 2012-09-19 | - |
dc.identifier.citation | Sood N, Chaudhary DK, Singh A, Rathore G. Monoclonal antibody to serum immunoglobulins of Clarias batrachus and its application in immunoassays. Gene. 2012 Dec 15;511(2):411-9. doi: 10.1016/j.gene.2012.09.044. Epub 2012 Sep 19. PubMed PMID: 23000018. | en_US |
dc.identifier.other | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/4881 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Serum immunoglobulins of Clarias batrachus (Cb-Ig) were purified by affinity chromatography using bovine serum albumin as capture ligand. Under reducing conditions in SDS-PAGE, Cb-Ig was composed of a heavy (H) chain (68.7 kDa) and two light (L) chains (27.4 and 26.3 kDa). Purified Cb-Ig was used to produce a monoclonal antibody (MAb) designated E4 MAb that belonged to IgG1 subclass. In Western blotting, this MAb showed binding to H chain of purified Cb-Ig and putative H chains in reduced sera of C. batrachus, Clarias gariepinus and Heteropneustes fossilis. However, no binding was observed with serum protein of Labeo rohita and Channa striata. Cross-reactivity of anti-Cb-Ig MAb was observed with serum of C. batrachus, C. gariepinus and H. fossilis in competitive ELISA. In immunoblotting of non-reduced Cb-Ig with E4 MAb, four bands assumed to be tetrameric, trimeric, dimeric and monomeric form were observed. In flow cytometric analysis of the gated lymphocytes, the number of surface Ig-positive (Ig +) cells in blood, spleen, kidney and thymus of C. batrachus was determined to be 50.1 ± 3.1, 55.1 ± 3.36, 42.4 ± 4.81 and 5.1 ± 0.89%, respectively, using E4 MAb. Ig + cells were also demonstrated in formalin-fixed paraffin embedded tissue sections of spleen, kidney, thymus and smears of blood mononuclear cells in indirect immunoperoxidase test. The developed MAb was employed to detect pathogen-specific immunoglobulins in the sera of C. batrachus immunized with killed Edwardsiella tarda, by an indirect ELISA. This monoclonal antibody can be useful tool in immunological research and assays. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | ELSEVIER | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Clarias batrachus | en_US |
dc.subject | Flow cytometry | en_US |
dc.subject | ELISA | en_US |
dc.subject | Immunoglobulin | en_US |
dc.subject | Immunoperoxidase test | en_US |
dc.subject | Monoclonal antibody | en_US |
dc.title | Monoclonal antibody to serum immunoglobulins of Clarias batrachus and its application in immunoassays | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Gene | en_US |
dc.publication.volumeno | 511 | en_US |
dc.publication.pagenumber | 411-419 | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | http://dx.doi.org/10.1016/j.gene.2012.09.044 | en_US |
dc.publication.naasrating | 8.98 | en_US |
Appears in Collections: | FS-NBFGR-Publication |
Files in This Item:
File | Description | Size | Format | |
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1-s2.0-S0378111912011250-main.pdf | 1.18 MB | Adobe PDF | View/Open |
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