KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/49651
Title: | Isolation and characterization of virulent Serratia marcescens associated with a disease outbreak in farmed ornamental fish, Poecilia reticulata in Kerala, India |
Other Titles: | Not Available |
Authors: | Arathi Dharmaratnam Raj kumar V. S. Basheer Neeraj Sood T. Raja Swaminathan J. K. Jena |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | Peninsular and Marine Fish Genetic Resources Centre of ICAR-National Bureau of Fish Genetic Resources, Lucknow Indian Council of Agricultural Research, Krishi Anusandhan Bhawan-II, Pusa, New Delhi |
Published/ Complete Date: | 2017-12-01 |
Project Code: | Not Available |
Keywords: | Bacterial fish diseases, Serratia marcescens Guppy Poecilia reticulata, |
Publisher: | ICAR - Central Marine Fisheries Research Institute |
Citation: | Arathi Dharmaratnam, Raj kumar, V. S. Basheer, Neeraj Sood, T. Raja Swaminathan* and J. K. Jena. 2017. Isolation and characterization of virulent Serratia marcescens associated with a disease outbreak in farmed ornamental fish, Poecilia reticulata in Kerala, India. Indian Journal of Fisheries, 64(4): 71-79. DOI: 10.21077/ijf.2017.64.4.71261-10 |
Series/Report no.: | Not Available; |
Abstract/Description: | Pathogenic strain of Serratia marcescens (NPSM-1) with multiple drug resistance was isolated from guppy Poecilia reticulata with clinical signs of fin rot and was confirmed by biochemical tests and 16S rRNA gene sequencing. The extra cellular proteins (ECP) of the bacteria exhibited marked cytotoxic activity in vitro on Cyprinus carpio koi fin (CCKF) cell line. The in vivo challenge studies confirmed that the isolate was highly pathogenic to fish when the fishes were injected with 1 x 104 CFU/fish and the same bacterium was re-isolated from infected fish, post-challenge. S. marcescens produced large zones of haemolysis on 10% sheep blood agar. The bacteria was found to carry virulence genes; extracellular metallo-protease gene (Pr596) and AHL synthase gene (SpnI). The bacterial isolate was tested to determine sensitivity against 16 antibiotics and was sensitive to only 5 viz., cefixime, chloramphenicol, ciprofloxacin, gentamycin and erythromycin. The study indicates that S. marcescens can cause disease in ornamental fish and the bacterium being a known human pathogen, may also cause infections in humans having direct contact with infected fishes. This is the first report describing S. marcescens as a pathogen of freshwater ornamental fish in India. |
Description: | Not Available |
ISSN: | 0970-6011 |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Indian Journal of Fisheires |
Journal Type: | Indian Journal of Fisheries is published quarterly by the Indian Council of Agricultural Research (ICAR) and original contributions in the field of fish and fisheries science will be considered for publication in the Journal. |
NAAS Rating: | 6.29 |
Impact Factor: | 0.50 |
Volume No.: | 64(4) |
Page Number: | 71-79 |
Name of the Division/Regional Station: | Peninsular and Marine Fish Genetic Resources Centre, Kochi |
Source, DOI or any other URL: | 10.21077/ijf.2017.64.4.71261-10 http://epubs.icar.org.in/ejournal/index.php/IJF/article/view/71261/32064 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/49651 |
Appears in Collections: | FS-NBFGR-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.