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http://krishi.icar.gov.in/jspui/handle/123456789/50385| Title: | APPLICATION OF SIMPLE SEQUENCE REPEAT (SSR) MARKERS IN DIVERSITY ANALYSIS OF TOBACCO TYPES |
| Other Titles: | Not Available |
| Authors: | K. PRABHAKARARAO K. SARALA T.G.K. MURTHY P.V. VENUGOPALA RAO V. KRISHNAMURTHY M. SUMA |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Central Tobacco Research Institute |
| Published/ Complete Date: | 2012-06-30 |
| Project Code: | Not Available |
| Keywords: | SSR MARKERS TOBACCO |
| Publisher: | Indian Society of Tobacco Science |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Tobacco is a high value cash crop with variety of end uses and also serving as a model plant in biotechnology with its broad genetic base. Assessment of genetic variation among different tobacco types and varieties will form the basis for various crop improvement programmes and genetic studies. Molecular markers in biological studies opened new possibilities for analyzing genetic diversity and determining the genetic relationships among and within the species. Microsatellite or Simple Sequence Repeat (SSR) markers are known for the reliable detection of genetic diversity, for their co-dominant nature and repeatability. The present study revealed the efficacy of 37 SSR markers in identifying the polymorphism among 48 varieties covering different tobacco types. The results revealed that 25 of them are potential polymorphic markers and were able to resolve the diversity among tobacco varieties. Analysis of genetic similarity coefficients based on SSR amplification data using clustering tools (UPGMA) grouped them into different clusters. Microsatellite markers provide sufficient resolution to distinguish among closely related tobacco types and it also projects their applicability in DNA fingerprinting. |
| Description: | Not Available |
| ISSN: | 0379 - 055X |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Tobacco Research |
| NAAS Rating: | 3.33 |
| Volume No.: | 38(1) |
| Page Number: | 29-33 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/50385 |
| Appears in Collections: | CS-CTRI-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| TR38(1)29-33.pdf | 263.12 kB | Adobe PDF | View/Open |
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