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http://krishi.icar.gov.in/jspui/handle/123456789/51629
Title: | A quick, easy and cost-effective in planta method to develop direct transformants in wheat |
Authors: | Avijit Tarafdar, Harinder Vishwakarma, S Gothandapani, Meenal Bhati, Koushik Biswas, Arul Prakash, Uttara Chaturvedi, Amolkumar U Solanke and Jasdeep Chatrath Padaria |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | NATIONAL INSTITUTE FOR PLANT BIOTECHNOLOGY |
Published/ Complete Date: | 2019-04-17 |
Project Code: | Not Available |
Keywords: | Indian bread wheat, Agrobacterium tumefaciens, In planta transformation, Apical meristem, Herbicide tolerance, CP4-EPSPS transgenic wheat |
Publisher: | SpringerLink |
Citation: | Tarafdar, A., Vishwakarma, H., Gothandapani, S. et al. A quick, easy and cost-effective in planta method to develop direct transformants in wheat. 3 Biotech 9, 180 (2019). https://doi.org/10.1007/s13205-019-1708-6 |
Abstract/Description: | Agrobacterium mediated in planta method was used to transform Indian elite wheat genotype HD2894 with herbicide-tolerant CP4-EPSPS (5-enolpyruvylshikimate-3-phosphate synthase) gene. The apical meristems of germinated seeds were targeted for introgression of transgene. The obtained T1 plants were screened by spraying 1% glyphosate and only positive transformants survived. The presence of transgene was also confirmed by PCR and Southern hybridization. Using this method, 3.07% transformation rate was observed. To identify transgenic lines carrying stably integrated CP4-EPSPS gene, the transgenic populations were screened in T3 generation using 1% glyphosate and lines with 100% survival were considered as homozygous. No significant morpho-physiological variations were observed within the transgenic lines as compared to non-transgenic plants. The present study resulted in herbicide-tolerant transgenic wheat and provides a valuable tool for development of wheat genetic transformation. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Article |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Biotech-3 |
NAAS Rating: | 7.8 |
Impact Factor: | 2.406 |
Volume No.: | 9 |
Page Number: | 180 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | https://doi.org/10.1007/s13205-019-1708-6 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/51629 |
Appears in Collections: | CS-NIPB-Publication |
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