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http://krishi.icar.gov.in/jspui/handle/123456789/52574| Title: | DNA barcoding Indian freshwater fishes |
| Other Titles: | Not Available |
| Authors: | Wazir Singh Lakra M Singh Mukunda Goswami A Gopalakrishnan K K Lal V Mohindra U K Sarkar P P Punia K V Singh J P Bhatt S Ayyappan |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR-Central Institute of Fisheries Education, Versova, Mumbai Department of Zoology and Biotechnology, Hemwati Nandan Bahuguna Garhwal University, Srinagar, Uttarakhand – 246174, India Indian Council of Agricultural Research, New Delhi, India ICAR-National Bureau of Fish Genetic Resources, Lucknow |
| Published/ Complete Date: | 2015-12-24 |
| Project Code: | Not Available |
| Keywords: | DNA barcode COI Genetic divergence Phylogeny |
| Publisher: | Taylor & Francis |
| Citation: | Lakra, W.S., Singh, M., Goswami, M., Gopalakrishnan, A., Lal, K.K., Mohindra, V., Sarkar, U.K., Punia, P.P., Singh, K.V., Bhatt, J.P. and Ayyappan, S. (2016) DNA barcoding Indian freshwater fishes, Mitochondrial DNA Part A, 27:6, 4510-4517, DOI: 10.3109/19401736.2015.1101540 |
| Series/Report no.: | Not Available; |
| Abstract/Description: | DNA barcoding is a promising technique for species identification using a short mitochondrial DNA sequence of cytochrome c oxidase I (COI) gene. In the present study, DNA barcodes were generated from 72 species of freshwater fish covering the Orders Cypriniformes, Siluriformes, Perciformes, Synbranchiformes, and Osteoglossiformes representing 50 genera and 19 families. All the samples were collected from diverse sites except the species endemic to a particular location. Species were represented by multiple specimens in the great majority of the barcoded species. A total of 284 COI sequences were generated. After amplification and sequencing of 700 base pair fragment of COI, primers were trimmed which invariably generated a 655 base pair barcode sequence. The average Kimura two-parameter (K2P) distances within-species, genera, families, and orders were 0.40%, 9.60%, 13.10%, and 17.16%, respectively. DNA barcode discriminated congeneric species without any confusion. The study strongly validated the efficiency of COI as an ideal marker for DNA barcoding of Indian freshwater fishes. |
| Description: | Not Available |
| ISSN: | 2470-1394 2470-1408 |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Mitochondrial DNA Part A - DNA Mapping, Sequencing & Analysis |
| Journal Type: | Mitochondrial DNA Part A publishes original high-quality manuscripts on physical, chemical, and biochemical aspects of mtDNA and proteins involved in mtDNA metabolism, and/or interactions. |
| NAAS Rating: | 7.07 |
| Impact Factor: | 1.514 |
| Volume No.: | 27(6) |
| Page Number: | 4510-4517 |
| Name of the Division/Regional Station: | Molecular Biology & Biotechnology |
| Source, DOI or any other URL: | https://doi.org/10.3109/19401736.2015.1101540 https://www.tandfonline.com/doi/abs/10.3109/19401736.2015.1101540?journalCode=imdn21 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/52574 |
| Appears in Collections: | FS-NBFGR-Publication |
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