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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/52615
Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 75. Hajira SK, Sundaram RM, Laha GS, Yugander A, Balachandran SM, Viraktamath BC, Sujatha K, Balachiranjeevi CH, Pranathi K, Anila M, Bhaskar S, Abhilash V, Mahadevaswamy HK, Kousik M, Dilipkumar T, Harika and Rekha G | en_US |
dc.date.accessioned | 2021-08-03T08:53:20Z | - |
dc.date.available | 2021-08-03T08:53:20Z | - |
dc.date.issued | 2016-05 | - |
dc.identifier.citation | S.K. Hajira, R.M. Sundaram, G.S. Laha, A. Yugander, S.M. Balachandran, B.C. Viraktamath, K. Sujatha, C.H. Balachiranjeevi, K. Pranathi, M. Anila, S. Bhaskar, V. Abhilash, H.K. Mahadevaswamy, M. Kousik, T. Dilip Kumar, G. Harika, G. Rekha,A Single-Tube, Functional Marker-Based Multiplex PCR Assay for Simultaneous Detection of Major Bacterial Blight Resistance Genes Xa21, xa13 and xa5 in Rice,Rice Science,Volume 23, Issue 3,2016,Pages 144-151,ISSN 1672-6308,https://doi.org/10.1016/j.rsci.2015.11.004 | en_US |
dc.identifier.issn | 1672-6308 | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/52615 | - |
dc.description | Not Available | en_US |
dc.description.abstract | In marker-assisted breeding for bacterial blight (BB) resistance in rice, three major resistance genes, viz., Xa21, xa13 and xa5, are routinely deployed either singly or in combinations. As efficient and functional markers are yet to be developed for xa13 and xa5, we have developed simple PCR-based functional markers for both the genes. For xa13, we designed a functional PCR-based marker, xa13-prom targeting the InDel polymorphism in the promoter of candidate gene Os8N3 located on chromosome 8 of rice. With respect to xa5, a multiplex-PCR based functional marker system, named xa5FM, consisting of two sets of primer pairs targeting the 2-bp functional nucleotide polymorphism in the exon II of the gene TFIIAɤ5 (candidate for xa5), has been developed. Both xa13-prom and xa5FM can differentiate the resistant and susceptible alleles for xa13 and xa5, respectively, in a co-dominant fashion. Using these two functional markers along with the already reported functional PCR-based marker for Xa21 (pTA248), we designed a single-tube multiplex PCR based assay for simultaneous detection of all the three major resistance genes and demonstrated the utility of the multiplex marker system in a segregating population. | en_US |
dc.description.sponsorship | Department of Biotechnology (DBT), Government of India (Grant Nos. BT/AB/FG-2 (PH-II)/2009 and BT/PR11705/AGR/02/646/2008) | en_US |
dc.language.iso | English | en_US |
dc.publisher | Not Available | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | bacterial blight | en_US |
dc.subject | xa5; xa13; Xa21 | en_US |
dc.subject | functional marker | en_US |
dc.subject | multiplex PCR | en_US |
dc.subject | Rice | en_US |
dc.title | A single-tube, functional marker-based multiplex PCR assay for simultaneous detection of major bacterial blight resistance genes in rice, Xa21, xa13 and xa5 | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Rice Science | en_US |
dc.publication.volumeno | 23(3) | en_US |
dc.publication.pagenumber | 144-151 | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | https://doi.org/10.1016/j.rsci.2015.11.004 | en_US |
dc.publication.authorAffiliation | ICAR::Indian Institute of Rice Research | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.naasrating | 9.16 | en_US |
dc.publication.impactfactor | 3.162 | en_US |
Appears in Collections: | CS-IIRR-Publication |
Files in This Item:
File | Description | Size | Format | |
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1-s2.0-S167263081630004X-main.pdf | 498.57 kB | Adobe PDF | View/Open |
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