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http://krishi.icar.gov.in/jspui/handle/123456789/52708| Title: | Genetic heterogeneity of non-O1 and non-O139 Vibrio cholerae isolates from shrimp aquaculture system: a comparison of RS-, REP- and ERIC-PCR fingerprinting approaches |
| Other Titles: | Not Available |
| Authors: | Rao, B.M. Surendran, P. K. |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Central Institute of Fisheries Technology |
| Published/ Complete Date: | 2010-07 |
| Project Code: | Not Available |
| Keywords: | black tiger shrimp ERIC-PCR genetic heterogeneity non-O1 and non-O139 Penaeus monodon REP-PCR RS-PCR Vibriocholerae |
| Publisher: | Wiley |
| Citation: | Rao, B. M. and Surendran, P.K. (2010) Genetic heterogeneity of non-O1 and non-O139 Vibriocholerae islates from shrimp aquaculture system: Acomparison of RS-, REP- and ERIC-PCRfingerprinting approaches, Lett. Appl. Microbiol., 51(1):65-74. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Aims: The genetic diversity of Vibrio cholerae isolated from black tiger shrimp (Penaeus monodon) aquaculture farms was determined using three PCR typing methods based on enterobacterial repetitive intergenic consensus (ERIC) sequences, ribosomal gene spacer (RS) sequence and repetitive extragenic palindromic (REP) sequences. Methods and Results: Non-O1 and non-O139 V. cholerae isolates were obtained from shrimp pond water, pond sediment, shrimp head and shrimp muscle. RS-PCR yielded fewer bands than REP-PCR and ERIC-PCR. Higher similarity was observed in RS-PCR (75–100%) than in REP-PCR (60–95%) and ERIC-PCR (40–95%). Conclusions: A 100% similarity between V. cholerae isolates was only noticed in RS-PCR. The choleratoxigenic V. cholerae (non-O1 and non-O139) showed greater genetic similarity with ctx-negative V. cholerae than among ctx-positive V. cholerae. Significance and Impact of the Study: The greater similarity of ctx-positive V. cholerae with ctx-negative V. cholerae isolates indicates that the ctx-positive strains (non-O1 and non-O139) might have originated from autochthonous V. cholerae in the aquatic niche. |
| Description: | Not Available |
| ISSN: | 0266-8254 |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Letters in Applied Microbiology |
| Journal Type: | International Journal |
| NAAS Rating: | 8.17 |
| Impact Factor: | 2.17 |
| Volume No.: | 51(1) |
| Page Number: | 65-74 |
| Name of the Division/Regional Station: | Microbiology, Fermentation and Biotechnology Division |
| Source, DOI or any other URL: | https://doi.org/10.1111/j.1472-765X.2010.02863.x |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/52708 |
| Appears in Collections: | FS-CIFT-Publication |
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