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http://krishi.icar.gov.in/jspui/handle/123456789/52836
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Anupama K, Pranathi K and Sundaram RM | en_US |
dc.date.accessioned | 2021-08-04T05:59:41Z | - |
dc.date.available | 2021-08-04T05:59:41Z | - |
dc.date.issued | 2020-05-01 | - |
dc.identifier.citation | Anupama K, Pranathi K and Sundaram RM (2020). Assessment of genetic purity of bulked‐seed of rice CMS lines using capillary electrophoresis. Electrophoresis (published online, doi: https://doi.org/10.1002/elps.201900429). | en_US |
dc.identifier.issn | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/52836 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Genetically pure cytoplasmic male sterile line (A-line) is essential to generate pure hybrid seeds in order to harness the yield heterosis in rice. Conventionally, seed purity testing is carried by grow-out test, which has many limitations. Seed purity assessments based on molecular markers reduce the time required for analysis significantly. However, it is very tedious as at least 200–400 seeds/seedlings are needed to be analyzed individually. An assay based on bulked-seed and molecular markers will be an ideal system. Keeping these points in view, in the present study, a co-dominant mitochondrial marker was used to test the purity of bulked parental line (A-line) seed utilizing CE system in a genetic analyzer. The results indicate that this method is very simple, accurate, and can be used to test purity of large number of samples rapidly in a cost-effective way compared to grow-out test and conventional molecular marker analysis. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Not Available | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | rice CMS lines | en_US |
dc.subject | genetic purity | en_US |
dc.subject | capillary electrophoresis | en_US |
dc.title | Assessment of genetic purity of bulked‐seed of rice CMS lines using capillary electrophoresis | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Article | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Electrophoresis | en_US |
dc.publication.volumeno | 41(20) | en_US |
dc.publication.pagenumber | 1749-1751 | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | https://doi.org/10.1002/elps.201900429 | en_US |
dc.publication.authorAffiliation | ICAR::Indian Institute of Rice Research | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.impactfactor | 3.081 | en_US |
Appears in Collections: | CS-IIRR-Publication |
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