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  1. KRISHI Publication and Data Inventory Repository
  2. Animal Science A4
  3. ICAR-National Dairy Research Institute D9
  4. AS-NDRI-Publication
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Please use this identifier to cite or link to this item: http://krishi.icar.gov.in/jspui/handle/123456789/53708
Title: Peripartum changes in the activity and expression of neutrophils may predispose to the postpartum occurrence of metritis in dairy cows
Other Titles: Not Available
Authors: Not Available
ICAR Data Use Licennce: http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf
Author's Affiliated institute: ICAR::National Dairy Research Institute
Published/ Complete Date: 2020-11-10
Project Code: Not Available
Keywords: Transition dairy cow Metritis Neutrophil molecule Myeloperoxidase CD14
Publisher: Not Available
Citation: Not Available
Series/Report no.: Not Available;
Abstract/Description: Metritis is a postpartum uterine pathology that causes a huge economic loss due to increased culling risk and impaired milk yield and reproduction in cows. The present study was carried out to study the changes in the activity and expression of blood neutrophils in crossbred dairy cows with and without metritis. Collection of blood samples was done at 􀀀 3, 􀀀 2 and 􀀀 1 weeks before calving, at calving and during the first day of metritis diagnosis in metritis group (n = 8) or at day 8–10 post calving in healthy group (n = 8). Neutrophils were studied for its percentage (microscopically), respiratory burst (nitro blue tetrazolium assay), myeloperoxidase (MPO) concentrations (sandwich ELISA) and expression of CXCR1, CXCR2, TLR2, TLR4, GRα, CD11b, CD14, CD25, CD44, CD47 and CD62L (RT-PCR). Immunocytochemistry was used to investigate MPO concentration and CD14 activity, and western blotting was used for estimating MPO. Although most of these parameters changed in the cows that developed metritis one week before calving, MPO and CD14 got altered much earlier. Myeloperoxidase concentrations and expression of CD14 were considerably lower starting from 􀀀 2 weeks before calving in cows that developed metritis compared to healthy cows. Further studies are warranted to study the possible use of MPO and CD14 to identify transition cows more vulnerable to develop metritis several weeks before disease occurrence.
Description: Not Available
ISSN: Not Available
Type(s) of content: Research Paper
Sponsors: Not Available
Language: English
Name of Journal: Not Available
Volume No.: Not Available
Page Number: Not Available
Name of the Division/Regional Station: Not Available
Source, DOI or any other URL: Not Available
URI: http://krishi.icar.gov.in/jspui/handle/123456789/53708
Appears in Collections:AS-NDRI-Publication

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