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http://krishi.icar.gov.in/jspui/handle/123456789/55156| Title: | Genotype differentiating RT-PCR and sandwich ELISA: handy tools in epidemiological investigation of foot and mouth disease |
| Other Titles: | Not Available |
| Authors: | Jajati Keshari Mohapatra Saravanan Subramaniam Chakradhar Tosh Divakar Hemadri Aniket Sanyal Tamilselvan Ramasamy Periyasamy Thaha Jamal Rasool |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Project Directorate on Foot and Mouth Disease |
| Published/ Complete Date: | 2007-07 |
| Project Code: | Not Available |
| Keywords: | FMD virusSerotype AGenotype differentiationRT-PCRSandwich ELISA |
| Publisher: | Elsevier |
| Citation: | Mohapatra JK, Subramaniam S, Tosh C, Hemadri D, Sanyal A, Periyasamy TR, Rasool TJ. Genotype differentiating RT-PCR and sandwich ELISA: handy tools in epidemiological investigation of foot and mouth disease. J Virol Methods. 2007 Jul;143(1):117-21. doi: 10.1016/j.jviromet.2007.02.008. Epub 2007 Apr 2. PMID: 17400298. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Years of molecular epidemiological surveillance has revealed co-circulation of two antigenically divergent genotypes of foot and mouth disease virus serotype A in India. Genotype differentiating RT-PCR and sandwich ELISA were developed as fast, cost-effective and user-friendly alternatives to 1D region based phylogeny for detection and differentiation of genotype VI and VII. The RT-PCR assay targeting 1D region was found to be more sensitive and authentic in distinguishing genotypes than sandwich ELISA. These assays promise to be reliable tools in the epidemiological investigation of foot and mouth disease in the country. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Journal of Virological Methods |
| NAAS Rating: | 7.79 |
| Volume No.: | 143 |
| Page Number: | 117-121 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | https://www.sciencedirect.com/science/article/abs/pii/S0166093407000857?via%3Dihub |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/55156 |
| Appears in Collections: | AS-PDFMD-Publication |
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