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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/55243
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Gupta,T., Kaur,H., Kapila,S.and R. Kapila | en_US |
dc.date.accessioned | 2021-08-12T06:16:58Z | - |
dc.date.available | 2021-08-12T06:16:58Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Gupta,T., Kaur,H., , Kapila,S. and Kapila,R. (2021) Lactobacillus fermentum (MTCC-5898) alleviates Escherichia coli induced inflammatory responses in intestinal epithelial cells by modulating immune genes and NF-κβ signalling. Journal of Applied Microbiology 10.1111/jam.15153 | en_US |
dc.identifier.issn | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/55243 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Aim Dietary intervention using probiotic bacteria has emerged as a promising preventive strategy in addressing foodborne infections or gastrointestinal disorders. This study investigated the immunomodulatory effects of Lactobacillus fermentum (MTCC-5898) on Escherichia coli-induced inflammatory responses in intestinal epithelial cells. Methods and Results The immune response of intestinal cells (Caco-2) in the presence of probiotic Lact. fermentum was determined during exclusion, competition and displacement of E. coli as the inflammatory agent. To achieve this objective, transcriptional modulation of key immune-related genes (cytokines, pattern recognition receptors and NF-κB), release of cytokines and nuclear translocation of the NF-κB subunit p-65 were studied. Expression of pro-inflammatory cytokines IL-8, TNF-α, IFN-ϒ and IL-23 was high in E. coli-exposed intestinal cells. However, overexpression of these E. coli-induced pro-inflammatory cytokines was prevented by Lact. fermentum during exclusion and competition assays. It also modulated the transcriptional expression of regulatory cytokines (IL-10 and TGF-β), pattern recognition receptors (TLR-2 and TLR-4) and genes associated with master inflammatory regulators (NF-κB and SIGIRR) to reduce E. coli-induced inflammation. The protective effect of Lact. fermentum was further confirmed by suppression of nuclear translocation of cytoplasmic NF-κB subunit (p-65). Conclusion Lactobacillus fermentum alleviated E. coli-induced inflammatory responses by modulating the NF-κB signalling besides pro-inflammatory and regulatory cytokines expression. Significance and Impact of the Study Lactobacillus fermentum holds significant promise as a potent nutraceutical that prevents and manages inflammatory gut-associated dysfunctions. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Wiley | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Lactobacillus fermentum | en_US |
dc.subject | Inflammatory responses | en_US |
dc.subject | Immune genes | en_US |
dc.title | Lactobacillus fermentum (MTCC-5898) alleviates Escherichia coli-induced inflammatory responses in intestinal epithelial cells by modulating immune genes and NF-κB signalling | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Journal of Applied Microbiology | en_US |
dc.publication.volumeno | Not Available | en_US |
dc.publication.pagenumber | Not Available | en_US |
dc.publication.divisionUnit | Animal Biochemistry Division | en_US |
dc.publication.sourceUrl | https://doi.org/10.1111/jam.15153 | en_US |
dc.publication.authorAffiliation | ICAR::National Dairy Research Institute | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.journaltype | Research | en_US |
dc.publication.naasrating | 9.07 | en_US |
dc.publication.impactfactor | 3.77 | en_US |
Appears in Collections: | AS-NDRI-Publication |
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