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http://krishi.icar.gov.in/jspui/handle/123456789/57202
Title: | Differential expression and localization of TRPV channels in the mature sperm of Anas platyrhynchos |
Other Titles: | Not Available |
Authors: | Rakesh Kumar Majhi 1,2 | Ashutosh Kumar 1,2 | Sunil C. Giri 3 | Chandan Goswami 1,2 |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | 1 School of Biological Sciences, National Institute of Science Education and Research, Bhubaneswar, India, 2 Homi Bhabha National Institute, Training School Complex, Anushakti Nagar, Mumbai, India, 3 Central Avian Research Institute, Bhubaneswar, India |
Published/ Complete Date: | 2020-10-01 |
Project Code: | Not Available |
Keywords: | TRPV1, TRPV2, TRPV3, TRPV4, TRPV5, TRPV6 |
Publisher: | WILEY-VCH-GmBH |
Citation: | Rakesh Kumar Majhi, Ashutosh Kumar , Sunil C. Giri, Chandan Goswami (2020) Differential expression and localization of TRPV channels in the mature sperm of Anas platyrhynchos. Reproduction in Domestic Animals 55(11), 1619-1628 |
Series/Report no.: | Not Available; |
Abstract/Description: | Sperm cells perform precise chemotactic and thermotactic movement which is crucial for fertilization. However, the key molecules involved in detection of different chemical and physical stimuli which guide the sperm during navigation are not well understood. Ca2+-signalling mediated by ion channels seem to play important role in motility and other fertility parameters. In this work, we explored the endogenous localization pattern of TRPV channels in the mature spermatozoa of avian species. Using sperm from white pekin duck (Anas platyrhynchos) as the representative avian model, we demonstrate that duck sperm endogenously express the thermosensitive channels TRPV1, TRPV2, TRPV3, TRPV4, and highly Ca2+-selective channels TRPV5 and TRPV6 in specific yet differential locations. All of these TRPV channels are enriched in the sperm tail, indicating their relevance in sperm motility. Interestingly, the TRPV3 and TRPV4 channels are present in the mitochondrial region. Calcium selective TRPV5 channel is exclusively present in sperm tail and is most abundant among the TRPV channels. This is the first report describing the endogenous presence of TRPV2 and TRPV3 channels in the sperm of any species. Using confocal imaging and super-resolution imaging, we demonstrate that though the TRPV channels are evolutionarily closely related, they have distinct localization pattern in the duck sperm, which could impact their role in fertilization. |
Description: | Not Available |
ISSN: | 0936-6768 |
Type(s) of content: | Article |
Sponsors: | Intramural funding to NISER (Department of Atomic Energy, Govt. of India), Grant/Award Number: 0000 |
Language: | English |
Name of Journal: | Reporduction in Domestic Animals |
Journal Type: | Scientific Research Journal |
NAAS Rating: | 7.64 |
Impact Factor: | 1.641 |
Volume No.: | 55(11) |
Page Number: | 1619-1628 |
Name of the Division/Regional Station: | Regional Station, ICAR-Directorate of Poultry Research, Rajendranagar, Hyderabad |
Source, DOI or any other URL: | DOI: 10.1111/rda.13822 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/57202 |
Appears in Collections: | AS-DPR-Publication |
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