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http://krishi.icar.gov.in/jspui/handle/123456789/5843| Title: | Identification, characterization, validation and cross-species amplification of genic-SSRs in Indian Mustard (Brassica juncea) |
| Other Titles: | Not Available |
| Authors: | Binay K. Singh Dwijesh C. Mishra Sushma Yadav Supriya Ambawat Era Vaidya Kishor U. Tribhuvan Arun Kumar Sujith Kumar Sanjeev Kumar K.K. Chaturvedi Reema Rani Prashant Yadav Anil Rai P.K. Rai V.V. Singh Dhiraj Singh |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Indian Institute of Agricultural Biotechnology ICAR::Indian Agricultural Statistics Research Institute ICAR::Directorate of Rapeseed and Mustard Research |
| Published/ Complete Date: | 2016-03-23 |
| Project Code: | Not Available |
| Keywords: | Brassica juncea Transcriptome Simple sequence repeats Cross-species amplification |
| Publisher: | Society for Plant Biochemistry and Biotechnology, Springer |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Brassica juncea is an economically important oilseed crop worldwide. It has limited genomic resources at present. We generated 47,962,057 expressed sequence reads which were assembled into 45,280 unigenes. A total of 4108 SSR loci (≥10 bp) were identified in these unigenes. Trinucleotide was the most frequent repeat unit (59.91 %) followed by di- (38.66 %), tetra - (0.71 %), hexa - (0.49 %) and pentanucleotide repeats (0.24 %). Primers were designed for 2863 SSR loci among which 460 were selected for primer synthesis. A total of 339 loci amplified successfully of which 134 (39.5 %) exhibited polymorphism among six B. juncea genotypes with PIC values ranging from 0.18 to 0.81. Further, 25 polymorphic SSRs were used for analysis of genetic variability in 25 genotypes of Brassicas and their wild relatives. Two to five alleles with PIC values 0.22–0.66 were detected at these loci. The dendrogram grouped the genotypes according to their known pedigree/systematic position. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Journal of Plant Biochemistry and Biotechnology |
| NAAS Rating: | 6.77 |
| Volume No.: | 25(4) |
| Page Number: | 410-420 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | https://link.springer.com/article/10.1007/s13562-016-0353-y https:// 10.1007/s13562-016-0353-y |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/5843 |
| Appears in Collections: | AEdu-IASRI-Publication |
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