KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/60766
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Dhankhar, J., Sharma, R. and Mann, B | en_US |
dc.date.accessioned | 2021-08-26T08:33:11Z | - |
dc.date.available | 2021-08-26T08:33:11Z | - |
dc.date.issued | 2016-08-21 | - |
dc.identifier.citation | Not Available | en_US |
dc.identifier.issn | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/60766 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Main objective of this study was to optimize the analytical procedures, especially extraction and saponification method for the better analysis of β-carotene in milk fat. Saponification method with suitable potassium hydroxide (KOH) concentration, use of antioxidant, saponification time, and temperature was optimized to get best extraction procedure for β-carotene . Analysis of β-carotene was carried out by using reversed phase high performance liquid chromatography (RP-HPLC) using C18 column. Chromatographic conditions best tested for the study involves methanol: tetrahydrofuran: water (MeOH: THF: H2 O). The effect of time temperature combination at 45̊C/30 min with saturated ascorbic acid had a pronounced effect on the β-carotene extraction from milk fat. Study also reveals that a concentration level of 10M KOH was best suited for the extraction of unsaponifiable. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Not Available | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | β-carotene Milk Saponification RP-HPLC Unsaponifiable | en_US |
dc.title | Optimization of various steps for RP-HPLC determination of carotene in milk fat. | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Not Available | en_US |
dc.publication.volumeno | Not Available | en_US |
dc.publication.pagenumber | Not Available | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | Not Available | en_US |
dc.publication.authorAffiliation | ICAR::National Dairy Research Institute | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
Appears in Collections: | AS-NDRI-Publication |
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