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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/60769
Title: | Immobilized aptamer on gold electrode senses trace amount of aflatoxin M1. |
Other Titles: | Not Available |
Authors: | Pandey, A.K.; Rajput, Y.S.; Sharma R. and Singh, D. |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::National Dairy Research Institute |
Published/ Complete Date: | 2017-11-13 |
Project Code: | Not Available |
Keywords: | Electrochemical aptasensor , Aflatoxin M1 , Aptamer, Square wave voltammetry |
Publisher: | Not Available |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | An electrochemical aptasensor for detection of trace amounts of aflatoxin M1 was developed. This required immobilization of aptamer on screen printed gold electrode comprising of working electrode, counter elec trode and reference electrode and was achieved by sequentially layering dithiodipropionic acid, streptavidin and biotinylated-tetraethylene glycol-aptamer. Immobi lization of aptamer was monitored by cyclic voltammetry. Peak current in square wave voltammogram was inversely related to logarithmic concentration of aflatoxin M1. Dynamic range of sensor was 1–105 ppt aflatoxin M1. Sensor can be regenerated by treating electrode with 10% sodium dodecyl sulfate or 40 mM tris-HCl (pH 8.0) con taining 10 mM ethylenediaminetetraacetic acid and 0.02% tween-20 |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Not Available |
Volume No.: | Not Available |
Page Number: | Not Available |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/60769 |
Appears in Collections: | AS-NDRI-Publication |
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