KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/60786
Title: | Separation methods for milk proteins on polyacrylamide gel electrophoresis: Critical analysis and options for better resolution. |
Other Titles: | Not Available |
Authors: | Sharma, N.; Sharma, R.; Rajput, Y.S.; Mann, B.; Singh, R. and Gandhi, K. |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::National Dairy Research Institute |
Published/ Complete Date: | 2021-03-01 |
Project Code: | Not Available |
Keywords: | polyacrylamide gel electrophoresis |
Publisher: | Elsevier |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | Over the years, a number of methodological variations in polyacrylamide gel electrophoresis (PAGE) have been attempted for the separation of milk proteins. Caseins are similar in size and differ very little in net charge from each other. Also, in heated milk, k-casein not only complexes with whey proteins but also undergoes self-polymerisation. These cause problems in the resolution of milk proteins and encouraged researchers to modify PAGE. Seven different types of PAGE, i.e., native-PAGE, urea-PAGE, reducing sodium dodecyl sulphate (SDS)-PAGE, non-reducing-SDS-PAGE, urea-SDS-PAGE, tricine-PAGE, and two dimensional electrophoresis (2DE), are reviewed with particular emphasis on the pattern of milk pro tein electrophoretograms, merits, demerits and applications. Although the literature suggests that SDS PAGE is increasingly used for separating milk proteins, other forms of PAGE have their own advantage. This review can act as a quick screening aid for researchers in selecting an appropriate type of PAGE for the separation of milk proteins for different purposes. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | International Dairy Journal |
NAAS Rating: | 8.51 |
Impact Factor: | 2.98 |
Volume No.: | 114 |
Page Number: | Not Available |
Name of the Division/Regional Station: | Dairy Chemistry |
Source, DOI or any other URL: | https://doi.org/10.1016/j.idairyj.2020.104920 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/60786 |
Appears in Collections: | AS-NDRI-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.