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http://krishi.icar.gov.in/jspui/handle/123456789/60849| Title: | Exploring the edible gum (galactomannan) biosynthesis and its regulation during pod developmental stages in clusterbean using comparative transcriptomic approach |
| Authors: | Sandhya Sharma, Anshika Tyagi, Harsha Srivastava, G. Ramakrishna, Priya Sharma, Amitha Mithra Sevanthi, Amolkumar U. Solanke, Ramavtar Sharma, Nagendra Kumar Singh, Tilak Raj Sharma Kishor Gaikwad |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR-National Institute for Plant Biotechnology, New Delhi, India ICAR-Central Arid Zone Research Institute, Jodhpur, India. DBT-National Agri-Food Biotechnology Institute, Mohali, India. |
| Published/ Complete Date: | 2021-02-17 |
| Keywords: | Galactomannan RGC-936, WRKY |
| Publisher: | Nature Research |
| Citation: | Sharma, S., Tyagi, A., Srivastava, H. et al. Exploring the edible gum (galactomannan) biosynthesis and its regulation during pod developmental stages in clusterbean using comparative transcriptomic approach. Sci Rep 11, 4000 (2021) |
| Series/Report no.: | Article number: 4000 (2021); |
| Abstract/Description: | Galactomannan is a polymer of high economic importance and is extracted from the seed endosperm of clusterbean (C. tetragonoloba). In the present study, we worked to reveal the stage-specific galactomannan biosynthesis and its regulation in clusterbean. Combined electron microscopy and biochemical analysis revealed high protein and gum content in RGC-936, while high oil bodies and low gum content in M-83. A comparative transcriptome study was performed between RGC-936 (high gum) and M-83 (low gum) varieties at three developmental stages viz. 25, 39, and 50 days after flowering (DAF). Total 209,525, 375,595 and 255,401 unigenes were found at 25, 39 and 50 DAF respectively. Differentially expressed genes (DEGs) analysis indicated a total of 5147 shared unigenes between the two genotypes. Overall expression levels of transcripts at 39DAF were higher than 50DAF and 25DAF. Besides, 691 (RGC-936) and 188 (M-83) candidate unigenes that encode for enzymes involved in the biosynthesis of galactomannan were identified and analyzed, and 15 key enzyme genes were experimentally validated by quantitative Real-Time PCR. Transcription factor (TF) WRKY was observed to be co-expressed with key genes of galactomannan biosynthesis at 39DAF. We conclude that WRKY might be a potential biotechnological target (subject to functional validation) for developing high gum content varieties. |
| ISSN: | 2045-2322 |
| Type(s) of content: | Research Paper |
| Sponsors: | ICAR-CRP on Genomics (NBFGR, Lucknow) |
| Language: | English |
| Name of Journal: | Scientific Reports |
| Journal Type: | Scientific Reports is an online peer-reviewed open access scientific mega journal published by Nature Research, covering all areas of the natural sciences. |
| NAAS Rating: | 10 |
| Impact Factor: | 4.379 |
| Volume No.: | Sci Rep 11, 4000 (2021) |
| Source, DOI or any other URL: | https://doi.org/10.1038/s41598-021-83507-3 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/60849 |
| Appears in Collections: | CS-NIPB-Publication |
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| File | Description | Size | Format | |
|---|---|---|---|---|
| Scientific reports.pdf | 5.39 MB | Adobe PDF | View/Open |
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